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Reversible capturing and voltammetric determination of circulating tumor cells using two-dimensional nanozyme based on PdMo decorated with gold nanoparticles and aptamer

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Abstract

A novel cytosensor was constructed for the ultrasensitive detection and nondestructive release of circulating tumor cells (CTCs) by combining Au nanoparticles-loaded two-dimensional bimetallic PdMo (2D Au@PdMo) nanozymes and electrochemical reductive desorption. The 2D Au@PdMo nanozymes possessed high-efficiency peroxidase-like activity and were assembled with an aptamer composed of a thiol-modified epithelial specific cell adhesion molecule (EpCAM) to strengthen CTCs adhesion. Moreover, the electrode surface was decorated with highly fractal Au nanostructures (HFAuNSs) composites due to the similarity in fractal nanostructure with the CTCs membrane to enhance the CTCs anchoring efficiency and release capability. The captured CTCs could be further efficiently dissociated and nondestructively released from the modified electrodes upon electrochemical reductive desorption. The designed cytosensor showed an excellent analytical performance, with a wide linear range from 2 to 1 × 105 cells mL−1 and low limit of detection (LOD) of 2 cells mL−1 (S/N = 3) at the working potential in the range  −0.6 to 0.2 V. A satisfactory CTCs release reaching a range of 93.7–97.4% with acceptable RSD from 3.55 to 6.41% and good cell viability was obtained. Thus, the developed cytosensor might provide a potential alternative to perform CTC-based liquid biopsies, with promising applications in early diagnosis of tumors.

Graphical abstract

Preparation and mechanism of desorption of the cytosensor based on 2D Au@PdMo nanozymes and electrochemical reductive desorption for the detection and release of CTCs. A Preparation procedure of the Apt/Au@PbMo bioconjugates. B Fabrication process of the sandwich-type cytosensor. C Electrochemical signal produced by the Au@PdMo nanozymes. D Mechanism of electrochemical reductive desorption for CTCs release

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Funding

This research was funded by the financial support from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202), the National Natural Science Foundation of China (81873980), the Natural Science Foundation Project of Chongqing (cstc2018jcyjAX0349), and the Zhejiang Provincial Natural Science Fund (LQ21H200005).

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  1. Wei Yang and Lu Fan contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China

    Wei Yang, Lu Fan, Haiping Wu, Junman Chen, Changjin Liu, Yurong Yan & Shijia Ding

  2. Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, 310014, China

    Wei Yang

  3. NMI Natural and Medical Sciences Institute, University of Tübingen, 72770, Reutlingen, Germany

    Lu Fan

  4. Department of Clinical Laboratory, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China

    Zhen Guo

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  1. Wei Yang
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Correspondence to Yurong Yan or Shijia Ding.

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Yang, W., Fan, L., Guo, Z. et al. Reversible capturing and voltammetric determination of circulating tumor cells using two-dimensional nanozyme based on PdMo decorated with gold nanoparticles and aptamer. Microchim Acta 188, 319 (2021). https://doi.org/10.1007/s00604-021-04927-6

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