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Paper-based sensor from pyrrolidinyl peptide nucleic acid for the efficient detection of Bacillus cereus

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Abstract

Bacillus cereus is one of the most common foodborne pathogens found in various kinds of staple foods such as rice and wheat. A rapid and accurate detection method for this pathogen is highly desirable for the sustainable production of relevant food products. While several classical and molecular-based detection methods are available for the identification of B. cereus, they suffered one or more limitations such as the requirement for a tedious and time-consuming process, less than ideal specificity, and the lack of portability. Herein, we developed the first paper-based sensing device that exhibits high species specificity with sufficiently low limit of detection for the visual detection of specific DNA sequences of B. cereus. The success is attributed to the strategic planning of fabrication in various dimensions including thorough bioinformatics search for highly specific genes, the use of the pyrrolidinyl peptide nucleic acid (PNA) probe whose selectivity advantage is well documented, and an effective PNA immobilization and DNA-binding visualization method with an internal cross-checking system for validating the results. Testing in rice matrices indicates that the sensor is capable of detecting and distinguishing B. cereus from other bacterial species. Hence, this paper-based sensor has potential to be adopted as a practical means to detect B. cereus in food industries.

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Funding

This work was supported by Thailand Science Research and Innovation (TSRI) (formerly the Thailand Research Fund, TRF) grant no. RGU6180001 (to TV) and MRG6280238 (to TP). NJ thanks the Science Achievement Scholarship of Thailand for his Ph.D. scholarship.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand

    Nuttapon Jirakittiwut, Tirayut Vilaivan & Thanit Praneenararat

  2. The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand

    Nuttapon Jirakittiwut, Tanutcha Patipong, Rungaroon Waditee-Sirisattha & Thanit Praneenararat

  3. Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand

    Nuttapon Jirakittiwut & Tirayut Vilaivan

  4. Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand

    Tanutcha Patipong & Rungaroon Waditee-Sirisattha

  5. Department of Physics, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Tawinan Cheiwchanchamnangij

Authors
  1. Nuttapon Jirakittiwut
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  2. Tanutcha Patipong
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  3. Tawinan Cheiwchanchamnangij
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  5. Tirayut Vilaivan
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  6. Thanit Praneenararat
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Correspondence to Thanit Praneenararat.

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Jirakittiwut, N., Patipong, T., Cheiwchanchamnangij, T. et al. Paper-based sensor from pyrrolidinyl peptide nucleic acid for the efficient detection of Bacillus cereus. Anal Bioanal Chem 413, 6661–6669 (2021). https://doi.org/10.1007/s00216-021-03633-9

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  • DOI: https://doi.org/10.1007/s00216-021-03633-9

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