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Genetic Diversity of Porcine Circovirus Subtypes from Aborted Sow Fetuses in Vietnam

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Abstract

Porcine circovirus type 3 (PCV3) is an emerging circovirus that is highly distributed among swine worldwide and associated with porcine dermatitis and nephropathy syndrome, reproductive failure, and multisystemic inflammation. Here, we investigated and characterized PCV3 from aborted fetuses in Vietnam. We found that the whole genomes of PCV3 collected in these Vietnamese pig farms share 98.4–99.45% sequence identity with reference PCV3 sequences. Several distinct mutation were identified in both the Rep protein and Cap protein of these strains. These strains were clustered into two distinct subtypes (3a1 and 3b). This study contributes to a better understanding of the molecular characteristics and genetic diversity of PCV3 in Vietnam.

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Data Availability

The complete genome sequences of the PCV3 strains identified in this study have been deposited in GenBank under the accession numbers MT847022, MT847024, and MT847031.

References

  1. Kwon T, Yoo SJ, Park CK, Lyoo YS (2017) Prevalence of novel porcine circovirus 3 in Korean pig populations. Vet Microbiol 207:178–180

    Article  Google Scholar 

  2. Palinski R, Pineyro P, Shang P, Yuan F, Guo R, Fang Y, Byers E, Hause BM (2017) A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J Virol. https://doi.org/10.1128/JVI.01879-16

    Article  PubMed  Google Scholar 

  3. Phan TG, Giannitti F, Rossow S, Marthaler D, Knutson TP, Li L, Deng X, Resende T, Vannucci F, Delwart E (2016) Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation. Virol J 13:184

    Article  Google Scholar 

  4. Shen H, Liu X, Zhang P, Wang L, Liu Y, Zhang L, Liang P, Song C (2018) Genome characterization of a porcine circovirus type 3 in South China. Transbound Emerg Dis 65:264–266

    Article  CAS  Google Scholar 

  5. Ouyang T, Niu G, Liu X, Zhang X, Zhang Y, Ren L (2019) Recent progress on porcine circovirus type 3. Infect Genet Evol 73:227–233

    Article  Google Scholar 

  6. Fu X, Fang B, Ma J, Liu Y, Bu D, Zhou P, Wang H, Jia K, Zhang G (2018) Insights into the epidemic characteristics and evolutionary history of the novel porcine circovirus type 3 in southern China. Transbound Emerg Dis 65:e296–e303

    Article  CAS  Google Scholar 

  7. Klaumann F, Correa-Fiz F, Franzo G, Sibila M, Nunez JI, Segales J (2018) Current knowledge on porcine circovirus 3 (PCV-3): a novel virus with a yet unknown impact on the swine industry. Front Vet Sci 5:315

    Article  Google Scholar 

  8. Chen N, Huang Y, Ye M, Li S, Xiao Y, Cui B, Zhu J (2019) Co-infection status of classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCV2 and PCV3) in eight regions of China from 2016 to 2018. Infect Genet Evol 68:127–135

    Article  Google Scholar 

  9. Jiang H, Wang D, Wang J, Zhu S, She R, Ren X, Tian J, Quan R, Hou L, Li Z, Chu J, Guo Y, Xi Y, Song H, Yuan F, Wei L, Liu J (2019) Induction of porcine dermatitis and nephropathy syndrome in piglets by infection with porcine circovirus type 3. J Virol 93(4):e02045

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Sun W, Wang W, Xin J, Cao L, Zhuang X, Zhang C, Zhu Y, Zhang H, Qin Y, Du Q, Han Z, Lu H, Zheng M, Jin N (2019) An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017. China. Virus Res 270:197663

    Article  CAS  Google Scholar 

  11. Stadejek T, Wozniak A, Milek D, Biernacka K (2017) First detection of porcine circovirus type 3 on commercial pig farms in Poland. Transbound Emerg Dis 64:1350–1353

    Article  CAS  Google Scholar 

  12. Collins PJ, McKillen J, Allan G (2017) Porcine circovirus type 3 in the UK. Vet Rec 181:599

    Article  CAS  Google Scholar 

  13. Tochetto C, Lima DA, Varela APM, Loiko MR, Paim WP, Scheffer CM, Herpich JI, Cerva C, Schmitd C, Cibulski SP, Santos AC, Mayer FQ, Roehe PM (2018) Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil. Transbound Emerg Dis 65:5–9

    Article  CAS  Google Scholar 

  14. Kedkovid R, Woonwong Y, Arunorat J, Sirisereewan C, Sangpratum N, Lumyai M, Kesdangsakonwut S, Teankum K, Jittimanee S, Thanawongnuwech R (2018) Porcine circovirus type 3 (PCV3) infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex (PRDC). Vet Microbiol 215:71–76

    Article  Google Scholar 

  15. Fux R, Sockler C, Link EK, Renken C, Krejci R, Sutter G, Ritzmann M, Eddicks M (2018) Full genome characterization of porcine circovirus type 3 isolates reveals the existence of two distinct groups of virus strains. Virol J 15:25

    Article  Google Scholar 

  16. Franzo G, Legnardi M, Hjulsager CK, Klaumann F, Larsen LE, Segales J, Drigo M (2018) Full-genome sequencing of porcine circovirus 3 field strains from Denmark, Italy and Spain demonstrates a high within-Europe genetic heterogeneity. Transbound Emerg Dis 65:602–606

    Article  CAS  Google Scholar 

  17. Nguyen VG, Chung HC, Huynh TML, Cao TBP, Vu NT, Le VT, Pham HQ (2018) Molecular characterization of novel porcine circovirus 3 (PCV3) in pig populations in the north of Vietnam. Arch Gene Genome Res 1(1):24–32

    Google Scholar 

  18. Guarino H, Goyal SM, Murtaugh MP, Morrison RB, Kapur V (1999) Detection of porcine reproductive and respiratory syndrome virus by reverse transcription-polymerase chain reaction using different regions of the viral genome. J Vet Diagn Investig 11:27–33

    Article  CAS  Google Scholar 

  19. Oh WT, Kim RY, Nguyen VG, Chung HC, Park BK (2017) Perspectives on the Evolution of Porcine Parvovirus. Viruses 9:196

    Article  Google Scholar 

  20. Larochelle R, Antaya M, Morin M, Magar R (1999) Typing of porcine circovirus in clinical specimens by multiplex PCR. J Virol Methods 80:69–75

    Article  CAS  Google Scholar 

  21. Ku X, Chen F, Li P, Wang Y, Yu X, Fan S, Qian P, Wu M, He Q (2017) Identification and genetic characterization of porcine circovirus type 3 in China. Transbound Emerg Dis 64:703–708

    Article  CAS  Google Scholar 

  22. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  CAS  Google Scholar 

  23. Nguyen NH, Do Tien D, Nguyen TQ, Nguyen TT, Nguyen MN (2021) Identification and whole-genome characterization of a novel porcine circovirus 3 subtype b strain from swine populations in Vietnam. Virus Genes. https://doi.org/10.1007/s11262-021-01844-x

    Article  PubMed  Google Scholar 

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Acknowledgements

We thank Declan Clarke (Yale University) for comments that greatly improved the manuscript. We are grateful for technical support that we received from the HanViet Veterinary Diagnosis Laboratory at Nong Lam University.

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Authors and Affiliations

  1. Department of Infectious Diseases and Veterinary Public Health Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Thu Duc district, Ho Chi Minh City, Vietnam

    Ngoc Hai Nguyen & Duy Tien Do

  2. Han-Viet Veterinary Diagnosis Laboratory, Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City, Vietnam

    Ngoc Hai Nguyen, Trung Quan Nguyen & Thuy Trang Nguyen

  3. Research Center for Genetics and Reproductive Health, School of Medicine, Vietnam National University Ho Chi Minh City, Linh Trung ward, Thu Duc district, Ho Chi Minh City, Vietnam

    Minh Nam Nguyen

Authors
  1. Ngoc Hai Nguyen
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  2. Duy Tien Do
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  3. Trung Quan Nguyen
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  4. Thuy Trang Nguyen
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  5. Minh Nam Nguyen
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Contributions

NHN, DDT, and MNN designed the study. TQN and TTN performed experiments. NHN and MNN analyzed the data. NHN, DDT, and MNN wrote the manuscript.

Corresponding authors

Correspondence to Ngoc Hai Nguyen or Minh Nam Nguyen.

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All authors have read the journal's policy on disclosures of potential conflicts of interest, and we declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Ethical Approval

The study was conducted in compliance with the institutional rules for the care and use of laboratory animals and using a protocol approved by the Ministry of Agriculture and Rural Development (MARD) Vietnam (TCVN 8402:2010).

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Nguyen, N.H., Do, D.T., Nguyen, T.Q. et al. Genetic Diversity of Porcine Circovirus Subtypes from Aborted Sow Fetuses in Vietnam. Curr Microbiol 78, 3751–3756 (2021). https://doi.org/10.1007/s00284-021-02641-3

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  • DOI: https://doi.org/10.1007/s00284-021-02641-3

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