Abstract
Hypoxia in water that caused by reduced levels of oxygen occurred frequently, due to the complex aquatic environment. Hypoxia tolerance for fish depends on a complete set of coping mechanisms such as oxygen perception and gene-protein interaction regulation. The present study examined the short-term effects of hypoxia on the brain in Takifugu rubripes. We sequenced the transcriptomes of the brain in T. rubripes to study their response mechanism to acute hypoxia. A total of 167 genes were differentially expressed in the brain of T. rubripes after exposed to acute hypoxia. Gene ontology and KEGG enrichment analysis indicated that hypoxia could cause metabolic and neurological changes, showing the clues of their adaptation to acute hypoxia. As the most complex and important organ, the brain of T. rubripes might be able to create a self-protection mechanism to resist or reduce damage caused by acute hypoxia stress.
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Data availability
All sequencing data were submitted to the Sequence Read Archive (SRA) public database in NCBI (www.ncbi.nlm.nih.gov/), under the accession code PRJNA645780. All other data included in this study are available upon request by contact with the corresponding author (Yang Liu).
Code availability
Python code for PCA was included in Supplementary Information File S12. Other codes used in this study are available upon request by contact with the corresponding author (Yang Liu).
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We are very grateful to Dalian Tianzheng Industrial Co. for providing experimental materials and sites.
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This work was supported by the National Key R&D Program of China (2018YFD0900301-10) and the China Agriculture Research System (CARS-47).
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M. Bao performed the experiments and sampling, initially analyzed the results, and drafted the manuscript. F. Shang further analyzed the experimental results, made graphs, and revised the paper. M. Bao and F. Shang contributed equally to this work. F. Liu, Z. Hu, S. Wang, X. Yang, Y. Yu, H. Zhang, C. Jiang, and J. Jiang participated in the experiment and sampling. Y. Liu and X. Wang wrote and reviewed the manuscript. All authors reviewed and approved the final manuscript.
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Bao, M., Shang, F., Liu, F. et al. Comparative transcriptomic analysis of the brain in Takifugu rubripes shows its tolerance to acute hypoxia. Fish Physiol Biochem 47, 1669–1685 (2021). https://doi.org/10.1007/s10695-021-01008-6
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DOI: https://doi.org/10.1007/s10695-021-01008-6