Abstract
A Gram-stain negative, non-flagellated, beige-pigmented, circular, catalase-positive, oxidase-positive bacterium, designated G4T, was isolated from gut microflora of top shell (Trochus maculatus Linnaeus) collected from Diwanggong market, Weihai, People’s Republic of China. The novel isolate was able to grow at 4–42 °C (optimum 25–33 °C), pH 7.0–9.0 (optimum 6.5–7.0) and with 0.0–11.0% NaCl (optimum 2.0–3.0%, w/v). Analysis of 16S rRNA gene sequence revealed that strain G4T shared the highest 16S rRNA gene sequence similarities with Oceaniglobus ichthyenteri YLY08T (96.6%), followed by Oceaniglobus indicus 1-19bT (95.3%). The genome of strain G4T, with 32 assembled contigs, was 4.5 Mb long with a G+C content of 65.3 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70% limit for species delineation. The average amino acid identity, average nucleotide identity and digital DNA–DNA genome hybridization relatedness between strain G4T and the closely related members of the genus Oceaniglobus, Oceaniglobus indicus1-19bT and Oceaniglobus ichthyenteri YLY08T were 71.3, 76.4 and 20.0%, and 75.0, 76.3 and 19.4%. The major cellular fatty acid was summed feature 8 (C18:1ω7c and/or C18:1ω6c). The sole respiratory quinone was Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyldimethylethanolamine. The results of phenotypical, phylogenetic and biochemical analyses indicated that strain G4T represents a novel species in genus Oceaniglobus within the family Rhodobacteraceae, for which the name Oceaniglobus trochenteri sp. nov. is proposed. The type strain is G4T (= MCCC 1K04356T = KCTC 82506T).
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Data availability
All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain G4T is MW555789 and the number for the whole genome sequence is JACNMM000000000. Supplementary figures and Supplementary tables are available with the online version of this paper.
Abbreviations
- AAI:
-
Average Amino Acid Identity
- ANI:
-
Average Nucleotide Identity
- Dddh:
-
Digital DNA–DNA Hybridization
- HPLC:
-
High Performance Liquid Chromatography
- HEPES:
-
N-(2Hydroxyethyl)Piperazine-N’-2-Ethanesulfonic Acid
- CAPSO:
-
3-Cyclohexylamino-2-Hydroxypropanesulfonic Acid Sodium Salt
- MES:
-
2-(N-Morpholino) ethane-sulfonic acid
- PIPES:
-
Piperazine-1,4-Bisethanesulfonic Acid
- Tricine:
-
N-[Tris(Hhydroxymethyl)Methyl] Glycine
- KCTC:
-
Korean Collection for Type Cultures
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- MEGA:
-
Molecular Evolutionary Genetics Analysis
- MCCC:
-
Marine Culture Collection of China
- PHB:
-
Poly-β-hydroxybutyrate
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Acknowledgements
We are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.
Funding
This work was supported by the National Natural Science Foundation of China (31700116), the Natural Science Foundation of Shandong Province (ZR2017MC019), the China Postdoctoral Science Foundation (2183), and the Key Science and Technology Program of Weihai (1070413421511).
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YXZ designed research and project outline. RY and ZC performed isolation, deposition, and identification. STY and MJZ performed genome analysis. ZC drafted the manuscript. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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Chen, Z., Yun, ST., Zhang, MJ. et al. Oceaniglobus trochenteri sp. nov., isolated from the gut microflora of top shell (Trochus maculatus Linnaeus). Arch Microbiol 203, 5613–5619 (2021). https://doi.org/10.1007/s00203-021-02543-9
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DOI: https://doi.org/10.1007/s00203-021-02543-9