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Genomic organization and hypoxia inducible factor responsive regulation of teleost hsp90β gene during hypoxia stress

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Abstract

Background

The physiological significance of a large family of heat-shock proteins (HSPs), comprised of the cytosolic HSP90A and the endoplasmic reticulum component of HSPB, is evident in prokaryotes and eukaryotes. The HSP90A is believed to play critical roles in diverse physiological functions of cell viability and chromosomal stability including stress management. Heightened abundance of hsp90β transcript was documented in Channa striatus, a freshwater fish, which is capable of surviving within an extremely hypoxic environment.

Methods and results

To better understand the mechanism of hsp90β gene expression, we investigated its genomic organization. Eleven exons were identified, including a long upstream intron with a remarkable similarity with human, but not with chicken counterpart. Dual-luciferase assays identified promoter activity in a 1366 bp 5′-flanking segment beyond the transcription initiation site. Examination detected a minimal promoter of 754 bp containing a TATA-box, CAAT-enhancer in addition to providing clues regarding other enhancer and repressor elements. The driving capability of this minimal promoter was further validated by its binding ability with TATA-box binding protein and the generation of GFP expressing transgenic zebrafish (F2). Further, deletion of an inverted HIF (hypoxia inducible factor) motif RCGTG (upstream of the TATA-box) dramatically reduced luciferase expression in a hypoxic environment (CoCl2 treated cultivable cells) and was identified as a cis-acting HIF responsive element, necessary for the hypoxia-induced expression.

Conclusions

The results obtained herein provide an insight regarding how hsp90β gene expression is controlled by HIF responsive element in teleost both during hypoxia stress management and normal physiological functions, and suggested that the hsp90β gene promoter could be used as a potential candidate for generating ornamental and food-fish transgenics.

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Acknowledgements

This work was funded by DBT and DST Women Scientist Scheme, Govt. of India. We thank Dr. Scott Steward-Tharp, Department of Pathology, University of Iowa for grammatical reviewing.

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Author notes

  1. Vemulawada Chakrapani

    Present address: Department of Pathology, 1080 Medical Laboratories, Carver College of Medicine, UIHC, University of Iowa, 500 Newton Road, Iowa City, IA, 52242, USA

Authors and Affiliations

  1. Fish Genetics & Biotechnology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India

    Hirak Kumar Barman, Shibani Dutta Mohapatra, Vemulawada Chakrapani, Subhajit Mondal, Binita Murmu, Meenati Manjari Soren & Kananbala Patra

  2. Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha, 751023, India

    Rajeeb Kumar Swain

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Contributions

HKB: overall designing and execution of the project, manuscript writing. SDM, SM, MMS and KP: gene characterization experiments, Writing—original draft, reviews and editing. VC, BM, RKS: transgenic experiments, maintenance of zebrafish and manuscript editing.

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Correspondence to Hirak Kumar Barman.

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Barman, H.K., Mohapatra, S.D., Chakrapani, V. et al. Genomic organization and hypoxia inducible factor responsive regulation of teleost hsp90β gene during hypoxia stress. Mol Biol Rep 48, 6491–6501 (2021). https://doi.org/10.1007/s11033-021-06657-7

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