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Design of an amperometric glucose oxidase biosensor with added protective and adhesion layers

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Abstract

Enzymes have demonstrated great potential in the development of advanced electroanalysis devices due to their unique recognition and catalytic properties. However, unsatisfactory stability and limited electron communication of traditional enzyme sensors seriously hinder their large-scale application. In this work, a simple and effective method is proposed to improve the stability of enzyme sensors by using sodium hyaluronate (SH) as a protective film, MXene-Ti3C2/Glucose oxidase (GOD) as the reaction layer, and chitosan (CS) /reduced graphene oxide (rGO) as the adhesion layer. Results demonstrate that the repeatability of the designed sensor increased by 73.3% after improving the adhesion between the reaction layer and the current collector and that its response ability was greatly enhanced. Moreover, the long-term stability of the electrode surface with SH protective film proved to be superior than that without protective film, which suggests that this design can effectively improve the overall performance of the enzyme biosensor. This work proposed a multi-tier synergistic approach for improving the reliability of enzyme sensors.

Our proposed protective and adhesion layer can greatly improve the stability of enzyme sensor and realize the rapid detection of glucose in serum sample.

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Funding

We appreciate the support from the National Natural Science Foundation of China (no. 21972111, 21773188), Fundamental Research Funds for the Central Universities(XDJK2019AA002, XDJK2019B052), Natural Science Foundation of Chongqing (cstc2018jcyjAX0714), Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Chongqing Key Laboratory for Advanced Materials and Technologies, and Venture and Innovation Support Program for Chongqing Overseas Returnees (cx2019073). We also thank Xuelian Yang, Qiuju Yang, and Yuanke Wu for helping with this work.

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Authors and Affiliations

  1. Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing, 400715, People’s Republic of China

    Rongwei Gao, Xuelian Yang, Qiuju Yang, Yuanke Wu & Shu-Juan Bao

  2. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, People’s Republic of China

    Feng Wang & Qingyou Xia

  3. Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715, People’s Republic of China

    Feng Wang & Qingyou Xia

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  1. Rongwei Gao
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Correspondence to Shu-Juan Bao.

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Gao, R., Yang, X., Yang, Q. et al. Design of an amperometric glucose oxidase biosensor with added protective and adhesion layers. Microchim Acta 188, 312 (2021). https://doi.org/10.1007/s00604-021-04977-w

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