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A Comparison of Genomic DNA Extraction Protocols in Artemisia annua L. for Large Scale Genetic Analyses Studies

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Abstract

Artemisia anuua L. is an important economic medicinal plant well known for its bioactive compound artemisinin that is used as an antimalarial drug. Extraction of high-quality genomic DNA suitable for restriction enzyme reactions and PCR amplifications is very difficult due to the presence of high amounts of secondary metabolites in A. anuua. This study compared efficiency of Fermentas genomic DNA purification kit (FGDK), phenol–Chloroform, cetyltrimethyl ammonium bromide (CTAB), sodium dodecyl sulfate and Sarkosyl nitrogen methods for extraction of DNA. The quality and quantity DNA samples were determined by physical appearance, agarose gel electrophoresis, spectrophotometer and PCR amplifications. The study used Inter retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite amplified polymorphism (REMAP) and start codon targeted-based primers. The results revealed that genomic DNA isolated by CTAB method was comparatively pure and produced clear polymorphic bands compared to the polymorphic bands noted on DNA extractions using other protocols.

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Acknowledgements

The authors gratefully acknowledge the laboratory facilities provided by Genomics & Molecular Breeding Lab, Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Iran. The research was financially supported by the University of Tabriz in co-operation with the Iran National Elites Foundation, under vote No. 102-1565.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

    Negar Valizadeh, Hossein Abbasi Holasou & Seyyed Abolghasem Mohammadi

  2. Center of Excellence in Cereal Molecular Breeding Iran, University of Tabriz, Tabriz, Iran

    Seyyed Abolghasem Mohammadi

  3. Department of Agronomy, Faculty of Agriculture, Ankara University, Ankara, Turkey

    Khalid Mahmood Khawar

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  1. Negar Valizadeh
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  2. Hossein Abbasi Holasou
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  3. Seyyed Abolghasem Mohammadi
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  4. Khalid Mahmood Khawar
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Correspondence to Negar Valizadeh.

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Valizadeh, N., Holasou, H.A., Mohammadi, S.A. et al. A Comparison of Genomic DNA Extraction Protocols in Artemisia annua L. for Large Scale Genetic Analyses Studies. Iran J Sci Technol Trans Sci 45, 1587–1595 (2021). https://doi.org/10.1007/s40995-021-01170-9

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  • DOI: https://doi.org/10.1007/s40995-021-01170-9

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