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Identification of Novel Differentially Expressing Long Non-Coding RNAs with Oncogenic Potential

  • GENOMICS. TRANSCRIPTOMICS
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Abstract

Recently, a wealth of data have been accumulating on the role of long non-coding RNAs (lncRNAs) in the fine-tuning of mRNA expression. Four new lncRNAs, namely, TMEM92-AS1, FAM222A-AS, TXLNB, and lnc-CCL28, were identified as differentially expressed in ovarian tumors using deep machine learning. The levels of lnc-CCL28 transcripts in both tumors and normal tissue samples were sufficient for further analysis by RT-PCR. In addition, the promising ovarian cancer biomarkers, lncRNAs LINC00152, NEAT1 and SNHG17 were added to RT-PCR analysis. For the first time, an increase in the level of lnc-CCL28 and SNHG17 lncRNAs was found in ovarian tumors, and the overexpression of LINC00152 and NEAT1 was confirmed. It seems that lnc-CCL28 is involved in carcinogenesis and, in particular, in ovarian cancer progression. Overexpression of LINC00152 and lnc-CCL28 was significantly associated with the later stages and metastasis.

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Funding

This work was supported by the Russian Science Foundation (grant no. 20-15-00368).

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, 125315, Moscow, Russia

    O. I. Brovkina, I. V. Pronina, L. A. Uroshlev, V. I. Loginov & E. A. Braga

  2. Federal Scientific and Clinical Center for Specialized Types of Medical Aid and Medical Technologies of FMBA of Russia, 115682, Moscow, Russia

    O. I. Brovkina

  3. Vavilov Institute of General Genetics, Russian Academy of Sciences, 117971, Moscow, Russia

    L. A. Uroshlev & M. V. Fridman

  4. Blokhin National Medical Research Center of Oncology of the Ministry of Health, Russian Federation, 115478, Moscow, Russia

    T. P. Kazubskaya, D. O. Utkin & N. E. Kushlinskii

Authors
  1. O. I. Brovkina
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  2. I. V. Pronina
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  3. L. A. Uroshlev
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  4. M. V. Fridman
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  5. V. I. Loginov
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  6. T. P. Kazubskaya
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  7. D. O. Utkin
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  8. N. E. Kushlinskii
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  9. E. A. Braga
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O.I.B. and I.V.P. made equal contributions.

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Correspondence to O. I. Brovkina.

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Conflict of interests. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in this work are in accordance with the ethical standards of the institutional committee on research ethics and the 1964 Declaration of Helsinki and its subsequent amendments or comparable standards of ethics. Written informed consent was obtained from all patients.

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Brovkina, O.I., Pronina, I.V., Uroshlev, L.A. et al. Identification of Novel Differentially Expressing Long Non-Coding RNAs with Oncogenic Potential. Mol Biol 55, 548–554 (2021). https://doi.org/10.1134/S0026893321020175

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  • DOI: https://doi.org/10.1134/S0026893321020175

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