Abstract—
Fundamental neurophysiological processes are often studied using Danio rerio fish as a model. A selective inhibitor of striatal-enriched protein tyrosine phosphatase (STEP) reduces serotonin metabolism in the D. rerio brain. Both STEP and serotonin are involved in the development of neurodegenerative behavioral disorders. Reduction or elevation of the serotonin level in the brain of mice caused by the administration of p-chlorophenylalanine or pargyline, respectively, results in a decrease in the level of рtpn5 mRNA in the striatum, рtpn5 being the gene encoding STEP. However, it has not been established whether this occurs in other organisms. We studied the effect of inhibitors of synthesis (p-chlorophenylalanine) and degradation (pargyline) of serotonin on the expression of the ptpn5 gene and the activity of STEP in the brain of D. rerio. The fish were placed in water containing p-chlorophenylalanine (2 mg/L) or pargyline (0.5 mg/L) for 72 hours, and control subjects were kept in aquarium water. The p-chlorophenylalanine treatment decreased the serotonin level in the brain fourfold, whereas pargyline increased the level of this transmitter sixfold. Both p-chlorophenylalanine and pargyline decrease STEP activity in the D. rerio brain, without affecting the level of the рtpn5 mRNA gene. Thus, interaction between STEP and the serotonin system is observed in both mammals and fish, which indicates the similarity of the regulation processes in vertebrates.
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REFERENCES
Kalueff A.V., Stewart A.M., Gerlai R. 2014. Zebrafish as an emerging model for studying complex brain disorders. Trends Pharmacol. Sci. 35, 63–75.
Stewart A.M., Braubach O., Spitsbergen J., Gerlai R., Kalueff A.V. 2014. Zebrafish models for translational neuroscience research: From tank to bedside. Trends Neurosci. 37, 264–278.
Goebel-Goody S.M., Baum M., Paspalas C.D., Fernandez S.M., Carty N.C., Kurup P., Lombroso P.J. 2012. Therapeutic implications for striatal-enriched protein tyrosine phosphatase (STEP) in neuropsychiatric disorders. Pharmacol. Rev. 64, 65–87.
Nguyen T.H., Liu J., Lombroso P.J. 2002. Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420. J. Biol. Chem. 277, 24274–24279.
Xu J., Kurup P., Bartos J.A., Patriarchi T., Hell J.W., Lombroso P.J. 2012. Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J. Biol. Chem. 287, 20942–20956.
Kurup P., Zhang Y., Xu J., Venkitaramani D.V., Haroutunian V., Greengard P., Nairn A.C., Lombroso P.J. 2010. A beta-mediated NMDA receptor endocytosis in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. J. Neurosci. 30, 5948–5957.
Sinyakova N.A., Kulikova E.A., Englevskii N.A., Kulikov A.V.2017. Effects of fluoxetine and potential antidepressant 8-trifluoromethyl 1,2,3,4,5-benzopentathiepin-6-amine hydrochloride (tc-2153) on behavior of Danio rerio fish in the novel tank test and brain content of biogenic amines and their metabolites. Bull. Exp. Biol. Med. 164 (5), 620–623.
Kulikova E.A., Bazhenova E.Y., Popova N.K., Khomenko T.M., Volcho K.P., Salakhutdinov N.F., Kulikov A.V. 2015. Effect of acute administration of 8-(trifluoromethyl)-1,2,3,4,5-benzopentathiepin-6-amine hydrochloride (TC-2153) on biogenic amines metabolism in mouse brain. Lett. Drug Design Discov. 12, 833–836.
Kulikov A.V., Tikhonova M.A., Kulikova E.A., Khomenko T.M., Korchagina D.V., Volcho K.P., Salakhutdinov N.F., Popova N.K.2011. Effect of a new potential psychotropic drug, 8-(trifluoromethyl)-1,2,3,4,5-benzopentathiepin-6-amine hydrochloride, on the expression of serotonin-related genes in the mouse brain. Mol. Biol. (Moscow). 45, 251–257.
Kulikova E.A., Khotskin N.V., Illarionova N.B., Sorokin I.E., Bazhenova E.Y., Kondaurova E.M., Volcho K.P., Khomenko T.M., Salakhutdinov N.F., Ponimaskin E., Naumenko V.S., Kulikov A.V. 2018. Inhibitor of striatal-enriched protein tyrosine phosphatase, 8-(trifluoromethyl)-1,2,3,4,5-benzopentathiepin-6-amine hydrochloride (TC-2153), produces antidepressant-like effect and decreases functional activity and protein level of 5-HT(2A) receptor in the brain. Neuroscience. 394, 220–231.
Kulikov A.V., Gainetdinov R.R., Ponimaskin E., Kalueff A.V., Naumenko V.S., Popova N.K. 2018. Interplay between the key proteins of serotonin system in SSRI antidepressants efficacy. Expert Opin. Ther. Targets. 22, 319–330.
Popova N.K., Naumenko V.S. 2019. Neuronal and behavioral plasticity: The role of serotonin and BDNF systems tandem. Expert Opin. Ther. Targets. 22, 227–239.
Walther D.J., Peter J.U., Bashammakh S., Hörtnagl H., Voits M., Fink H., Bader M. 2003. Synthesis of serotonin by a second tryptophan hydroxylase isoform. Science. 299, 76.
Popova N.K., Kulikov A.V. 2010. Targeting tryptophan hydroxylase 2 in affective disorder. Expert Opin. Ther. Targets. 14, 1259–1271.
Kulikov A.V., Popova N.K. 2015. Tryptophan hydroxylase 2 in seasonal affective disorder: underestimated perspectives? Rev. Neurosci. 26, 679–690.
Kulikova E.A., Kulikov A.V. 2019. Tryptophan hydroxylase 2 as a therapeutic target for psychiatric disorders: Focus on animal models. Expert Opin. Ther. Targets. 23, 655–667.
Shih J.C., Thompson R.F. 1999. Monoamine oxidase in neuropsychiatry and behavior. Am. J. Hum. Genet. 65, 593–598.
Allen D.L., Renner K.J., Luine V.N. 1993. Pargyline-induced increase in serotonin levels: Correlation with inhibition of lordosis in rats. Pharmacol. Biochem. Behav. 45, 837–841.
Edmondson D.E., Mattevi A., Binda C., Li M., Hubálek F. 2004. Structure and mechanism of monoamine oxidase. Curr. Med. Chem. 11, 1983–1993.
Kulikova E.A., Fursenko D.V., Bazhenova E.Yu., Kulikov A.V. 2020. Pargyline and p-chlorophenylalanine decrease expression of Ptpn5 encoding striatal-enriched protein tyrosine phosphatase (STEP) in the mouse striatum. Mol. Biol. (Moscow). 54, 274–280.
Khotskin N.V., Plyusnina A.V., Kulikova E.A., Bazhenova E.Y., Fursenko D.V., Sorokin I.E., Kolotygin I., Mormede P., Terenina E.E., Shevelev O.B., Kulikov A.V. 2019. On association of the lethal yellow (AY) mutation in the agouti gene with the alterations in mouse brain and behavior. Behav. Brain Res. 359, 446–456.
Kulikov A.V., Naumenko V.S., Voronova I.P., Tikhonova M.A., Popova N.K. 2005. Quantitative RT-PCR assay of 5-HT1A and 5-HT2A serotonin receptor mRNAs using genomic DNA as an external standard. J. Neurosci. Methods. 141, 97‒101.
Paul S., Snyder G.L., Yokakura H., Picciotto M.R., Nairn A.C., Lombroso P.J. 2000. The Dopamine/D1 receptor mediates the phosphorylation and inactivation of the protein tyrosine phosphatase STEP via a PKA-dependent pathway. J. Neurosci. 20, 5630–5638.
Barnes N.M., Sharp T. 1999. A review of central 5-HT receptors and their function. Neuropharmacology. 38, 1083–1152.
Pytliak M., Vargova V., Mechirova V., Felsoci M. 2011. Serotonin receptors—from molecular biology to clinical applications. Physiol. Res. 60, 15–25.
Panula P., Chen Y.C., Priyadarshini M., Kudo H., Semenova S., Sundvik M., Sallinen V. 2010. The comparative neuroanatomy and neurochemistry of zebrafish CNS systems of relevance to human neuropsychiatric diseases. Neurobiol. Dis. 40, 46–57.
Gaspar P., Lillesaar C. 2012. Probing the diversity of serotonin neurons. Philos. Trans R. Soc. Lond. B. 67, 2382–2394.
Borsini F. 1995. Role of the serotonergic system in the forced swimming test. Neurosci. Biobehav. Rev. 19, 377‒395.
Stewart A.M., Cachat J., Gaikwad S., Robinson K.S., Gebhardt M., Kalueff A.V. 2013. Perspectives on experimental models of serotonin syndrome in zebrafish. Neurochem. Int. 62, 893–902.
Maximino C., Puty B., Benzecry R., Araújo J., Lima M.G., de Jesus Oliveira Batista E., Renata de Matos Oliveira K., Crespo-Lopez M.E., Herculano A.M. 2013. Role of serotonin in zebrafish (Danio rerio) anxiety: Relationship with serotonin levels and effect of buspirone, WAY 100635, SB 224289, fluoxetine and para-chlorophenylalanine (pCPA) in two behavioral models. Neuropharmacology. 71, 83–97.
ACKNOWLEDGEMENTS
The authors are grateful to the fellows of the laboratory of physiologically active substances N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, for kindly granting the TC-2153 substance.
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The study was supported by Budgetary project no. 0259-2021-0015.
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Statement on the welfare of animals. All procedures performed in this work correspond to the ethical standards of the institutional committee for research ethics and the Helsinki Declaration of 1964 and its following changes or comparable ethical norms.
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Abbreviations: 5-HT, serotonin; ptpn5, STEP protein-encoding gene; pol2e, DNA-dependent RNA polymerase; STEP, Striatal-Enriched protein tyrosine Phosphatase; MAO, monoamine oxidase; TPH2, tryptophanhydroxilase 2.
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Kulikova, E.A., Fursenko, D.V., Bazhenova, E.Y. et al. Decrease in the Activity of Striatal-Enriched Protein-Tyrosine-Phosphatase (STEP) in the Brain of Danio rerio Treated with p-Chlorophenylalanine and Pargyline. Mol Biol 55, 604–609 (2021). https://doi.org/10.1134/S0026893321020254
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DOI: https://doi.org/10.1134/S0026893321020254