Abstract
Cromileptes altivelis, humpback grouper, belongs to the family Epinephelidae and is one popular farmed fish species because of its high economic value and ornamental value. However, more and more diseases outbreaks have been reported with C. altivelis aquaculture. Today, a new brain cell line of C. altivelis (named CAB) was established and characterized. Our results showed that CAB cells were suitable for growth at 26 °C in L-15 medium supplemented with 15% fetal bovine serum (FBS). The results of 18S rRNA gene sequencing confirmed that CAB cell line was derived from C. altivelis. Moreover, chromosomal aneuploidy was observed in CAB cells, and the modal chromosome number of CAB cells was 48 by chromosome analysis. In addition, CAB cells could transfect pEGFP-N3 plasmid with high transfection efficiency, indicating that CAB cell line has the potential to investigate the function of exogenous genes in vitro. Furthermore, the bacterial susceptibility results suggested that CAB cells were susceptive to Vibrio harveyi and Edwardsiella tarda. And, heavy metals (Hg, Cd, and Cu) were toxic to the CAB cells, and the toxic effect was dose-dependent. In summary, the CAB cell line could be a powerful tool in vitro to study functional genes and has the potential application in bacterial susceptibility and toxicology.
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This research was supported financially by the National Natural Science Foundation of China (No.42066007) and Natural Science Foundation of Hainan Province (320QN212).
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Yixuan Liu: formal analysis; writing, original draft preparation. Caoying Wei: formal analysis, data curation, software, visualization. Zhiru Liu: project administration. Zhenjie Cao: conceptualization; methodology; writing, reviewing and editing. Yun Sun: supervision; writing, reviewing and editing. Yongcan Zhou: data curation. Shifeng Wang: validation. Weiliang Guo: editing.
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Animal care was performed conformity with NIH guidelines (NIH Pub. No. 85e23, revised 1996) and was approved by Animal Care and Use Committee of the Hainan University.
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Liu, Y., Wei, C., Liu, Z. et al. Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility. Fish Physiol Biochem 47, 1645–1658 (2021). https://doi.org/10.1007/s10695-021-01006-8
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DOI: https://doi.org/10.1007/s10695-021-01006-8