Abstract
The effects of mono- and disubstituted phosphates on the photolysis reaction of reduced nicotinamide adenine dinucleotide (NADH) in a frozen (77 K) aqueous solution were studied by EPR. The dependence of the yield of the paramagnetic intermediates of the reaction, that is, the NADH+ cation radical and atomic hydrogen, on the concentration of phosphates was determined. The results were interpreted from the point of view of the basicity and electron acceptor properties of phosphates. Electron spectroscopy data revealed a significant acceleration of auto-oxidation and photoinduced oxidation of NADH under normal conditions (20°C, aqueous medium, phosphate buffer, pH 6.3). The rate of the dark reaction was directly proportional to the total concentration of orthophosphates and hydrophosphates and was insensitive to oxygen. On the basis of experiments with a nitroxyl trap, the conclusion was made that the reaction is not of a radical nature.
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The work was carried out with budget funding (FANO project no. 0082-2014-0009, state registration no. AAAA-A17-117040610309-0).
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Translated by E. Puchkov
Abbreviations: TEMPOL—4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl; Pi—mono- and disubstituted phosphates.
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Brzevskaya, O.N., Degtyarev, E.N. & Kholuiskaya, S.N. The Role of Phosphate Anions in Autoinduced and Photoinduced Oxidation of NADH. BIOPHYSICS 66, 379–384 (2021). https://doi.org/10.1134/S0006350921030027
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DOI: https://doi.org/10.1134/S0006350921030027