Abstract
A comparative kinetic analysis of albino rat brain synaptic and kidney plasma membrane fraction Na,K-ATPase was performed to comprehend the different levels of sensitivity of these fractions to the neurotransmitter noradrenaline. Noradrenaline (NA) inhibits the rat brain synaptic membrane Na,K-ATPase, changes the stoichiometry of Na+ and K+ and shifts the enzyme system from an MgATP to an Mg2+ dependent cycle. While the kidney plasma membrane fraction Na,K-ATPase is not sensitive to noradrenaline. To investigate the mechanism underlying this difference, we studied enzyme velocity dependence on the concentration of Mg2+. The 1/V = f(Mg2+) function has shown different kinetic features for the synaptic and kidney plasma membrane Na,K-ATPase. With the addition of ethylene glycol tetraacetic acid (EGTA) to the reaction medium the geometric form of 1/V = f(Mg2+) function is affected differently. We thereafter measured the essential activator number for Na+ and K+ with, in excess Mg2+. The results of these experiments reveal that, contrary to the synaptic membrane Na,K-ATPase, the kidney plasma membrane fraction Na,K-ATPase does not possess an Mg2+ dependent cycle and noradrenaline exhibits different modulatory effects on the enzyme system.
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The rats experienced no suffering prior to death, as their death was caused by decapitation. All experiments were approved by the animal care and use committee at the I. Beritashvili Center of Experimental Biomedicine.
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Chkadua, G., Nozadze, E., Tsakadze, L. et al. Some Kinetic Features of Na,K-ATPase and Sensitivity to Noradrenaline. Cell Biochem Biophys 80, 23–29 (2022). https://doi.org/10.1007/s12013-021-01032-6
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DOI: https://doi.org/10.1007/s12013-021-01032-6