Abstract
This study aimed to investigate the antioxidant and protective effect of E. persicus leaf hydroalcoholic extract (EPE) in preventing gastric ulcers induced by ethanol in rats. Wistar rats weighing 180–220 g were randomly divided into five groups. These groups included negative control (normal) group, positive control (ethanolic) group, comparative control (ranitidine recipient) group, group recipient the dose of 250 mg/kg plant extract, and group recipient the dose of 500 mg/kg plant extract. One hour after gavage of the drug and extract, the gastric ulcer was induced by feeding 1 ml of 96% ethanol to each animal except the rats of the negative control group. After one hour, the rats were killed, and their stomachs were separated. Then, the gastric Ulcer index (UI), pH, oxidative stress parameters, and histopathological changes in the stomach of all groups were measured. Pre-treatment of ethanol-induced rats with the EPE reduced (P < 0.05) the ulcer index and gastric juice pH, compared to ethanolic group rats. Furthermore, pre-treatment with EPE at a dose-dependent manner, alleviated the gastric oxidative stress injury in rats through increase the activity of CAT, tissue NO· and GSH levels. EPE also was able to decrease the levels of ROS, MDA, PCO and serum NO·. According to the results, it can be concluded that pre-treatment with EPE prevents the formation of gastric ulcers caused by ethanol, which can be attributed to the antioxidant activity of plant polyphenols compounds.
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Abbreviations
- EPE:
-
Eremurus persicus (Jaub & Spach) Boiss Extract
- RAN:
-
Ranitidine
- FCR:
-
Folin–Ciocalteu’s reagent
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- FTIR:
-
Fourier-transform infra-red spectroscopy
- PBS:
-
Phosphate-buffered saline
- DCF:
-
2,7-Dichlorofluorescein
- NO· :
-
Nitric oxide
- MDA:
-
Malondialdehyde
- PCO:
-
Protein carbonyl
- GSH:
-
Glutathione
- NSAIDs:
-
Nonsteroidal anti-inflammatory drugs
- ROS:
-
Reactive oxygen species
- CAT:
-
Catalase
- H2O2 :
-
Hydrogen peroxide
- iNOS:
-
Inducible nitric oxide synthase
- eNOS:
-
Endothelial nitric oxide synthase
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The authors appreciate the financial support of this investigation by Lorestan University.
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This study was supported by a grant from the Faculty of Basic Sciences, Lorestan University. The founding sponsor had no role in the study design, performance, data collection and analysis, decision to publish, or preparation/writing of the manuscript.
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MB conducted the experiments and wrote the manuscript and all images were drawn. SB participates in the design and interpretation of the studies, and the review of the manuscript. OD participated in the preparation of tissue samples and pathological analysis.
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Beiranvand, M., Bahramikia, S. & Dezfoulian, O. Evaluation of antioxidant and anti-ulcerogenic effects of Eremurus persicus (Jaub & Spach) Boiss leaf hydroalcoholic extract on ethanol-induced gastric ulcer in rats. Inflammopharmacol 29, 1503–1518 (2021). https://doi.org/10.1007/s10787-021-00868-x
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DOI: https://doi.org/10.1007/s10787-021-00868-x