1887

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Volume 70, Issue 8

The effect of probiotic supplementation on the gut microbiota of preterm infants Open Access

Abstract

Probiotic supplementation of preterm infants may prevent serious morbidities associated with prematurity.

To investigate the impact of probiotic supplementation on the gut microbiota and determine factors associated with detection of probiotic species in the infant gut.

Probiotic supplementation increases the long-term colonization of probiotic species in the gut of preterm infants.

Longitudinal stool samples were collected from a cohort of very preterm infants participating in a blinded randomized controlled trial investigating the impact of probiotic supplementation (containing subsp. BB-02, subsp. BB-12 and TH-4) for prevention of late-onset sepsis. The presence of subsp. , subsp. and was determined for up to 23 months after supplementation ended using real-time PCR. Logistic regression was used to investigate the impact of probiotic supplementation on the presence of each species.

Detection of subsp. [odds ratio (OR): 53.1; 95 % confidence interval (CI): 35.6–79.1; < 0.001] (OR: 89.1; 95 % CI: 59.0–134.5; < 0.001) and (OR: 5.66; 95 % CI: 4.35–7.37; < 0.001) was increased during the supplementation period in infants receiving probiotic supplementation. Post-supplementation, probiotic-supplemented infants had increased detection of subsp. (OR: 2.53; 95 % CI: 1.64–3.90; < 0.001) and subsp. (OR: 1.59; 95 % CI: 1.05–2.41; =0.030). Commencing probiotic supplementation before 5 days from birth was associated with increased detection of the probiotic species over the study period ( subsp. infantis OR: 1.20; subsp. lactis OR: 1.28; OR: 1.45).

Probiotic supplementation with subsp. BB-02, subsp. BB-12 and TH-4 enhances the presence of probiotic species in the gut microbiota of very preterm infants during and after supplementation. Commencing probiotic supplementation shortly after birth may be important for improving the long-term colonization of probiotic species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bifidobacterium animalis subsp. lactis , Bifidobacterium longum subsp. infantis , gut microbiota , preterm infants , probiotics and Streptococcus thermophilus
Funding
This study was supported by the:
  • The Angior Family Foundation, Melbourne, Australia
    • Principle Award Recipient: SuzanneM Garland
  • The Royal Women's Hospital Foundation
    • Principle Award Recipient: SuzanneM Garland
  • National Health and Medical Research Council (Award 454629)
    • Principle Award Recipient: SuzanneM Garland
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-08-25
2024-04-23
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The effect of probiotic supplementation on the gut microbiota of preterm infants
J. Med. Microbiol. 70, 001403 (2021); https://doi.org/10.1099/jmm.0.001403
/content/journal/jmm/10.1099/jmm.0.001403
/content/journal/jmm/10.1099/jmm.0.001403
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