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Volume 70, Issue 8

Genomic epidemiology of the opportunistic pathogen from companion dogs Open Access

Abstract

(formerly subsp. ) is a common commensal and opportunistic pathogen of companion dogs. It carries a range of antimicrobial resistance genes and is an occasional zoonotic pathogen.

Despite the potential insight offered by genome sequencing into the biology of , few genomes are currently available for study.

To sequence and analyse genomes to improve understanding of this organism’s molecular epidemiology, antimicrobial resistance and bacterium–host interactions.

Twenty-five genomes of clinical isolates collected at a veterinary referral hospital in Scotland, UK, were sequenced with Illumina technology. These genomes were analysed by a series of bioinformatics tools along with 16 previously sequenced genomes.

Phylogenetic comparison of the 41 genomes shows that the current phylogeny is dominated by clades of closely related isolates, at least one of which has spread internationally. Ten of the 11 methicillin-resistant genomes in this collection of 41 encoded the promoter and gene mutations that are predicted to render the isolates susceptible to penicillins in the presence of clavulanic acid, a feature only described to date in methicillin-resistant . Seven such isolates were from the current study and, in line with the genome-based prediction, all were susceptible to amoxicillin/clavulanic acid shared very few highly conserved virulence-associated genes with , another common commensal and opportunistic canine pathogen.

The availability of a further 25 genome sequences from clinical isolates will aid in better understanding the epidemiology, bacterial–host interactions and antimicrobial resistance of this opportunistic pathogen.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): coagulase-positive staphylococci , methicillin resistance , Staphylococcus coagulans and veterinary microbiology
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-08-25
2024-04-19
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Genomic epidemiology of the opportunistic pathogen Staphylococcus coagulans from companion dogs
J. Med. Microbiol. 70, 001407 (2021); https://doi.org/10.1099/jmm.0.001407
/content/journal/jmm/10.1099/jmm.0.001407
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