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A switchable and signal-amplified aptasensor based on metal organic frameworks as the quencher for turn-on detection of T-2 mycotoxin

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Abstract

A simple and low-cost fluorescence aptasensor was developed for rapid and sensitive signal amplification detection of T-2 mycotoxin (T-2). Dual-terminal-fluorescein amidite (FAM)–labeled aptamer (D-aptamer) acted as a recognition element and signal indicator. The metal organic frameworks (MOFs) of N, N′-bis(2-hydroxyethyl)dithiooxamidato copper (II) (H2dtoaCu) were as the quencher. The D-aptamer was initially adsorbed to the surface of H2dtoaCu, leading to efficient quenching of the aptasensor. Upon addition of T-2, the D-aptamer underwent a conformation change to form the T-2/T-2 aptamer complex, which induced the signaling probe to be released from the H2dtoaCu surface. Thus, the fluorescence intensity (FL) of the D-aptamer was recovered. Versus the single-terminal-FAM-labeled aptamer (S-aptamer), the D-aptamer showed a lower detection limit of 0.39 ng/mL. The aptasensor was also successfully applied to detect T-2 in corn and wheat samples with good recoveries.

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Acknowledgements

This work was supported by the Key Technologies Research and Development Program of China (2018YFC1604100), National Natural Science Foundation of China (21904064, 21878154), and Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJB150028).

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Authors and Affiliations

  1. College of Food and Pharmaceutical Engineering, Nanjing Normal University, Wenyuan Road, Nanjing, 210023, People’s Republic of China

    Xinliu Tan, Weidao Yu, Yuwen Wang, Ping Song, Qing Xu & Yaqiong Yang

  2. College of Biological and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu, 211816, People’s Republic of China

    Xinliu Tan & Dengming Ming

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  1. Xinliu Tan
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  2. Weidao Yu
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Contributions

Xinliu Tan: methodology, investigation, roles/writing—original draft

Weidao Yu: investigation

Yuwen Wang: funding acquisition

Ping Song: supervision

Qing Xu: funding acquisition

Dengming Ming: conceptualization

Yaqiong Yang: visualization, writing—review and editing, funding acquisition

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Correspondence to Yaqiong Yang.

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Tan, X., Yu, W., Wang, Y. et al. A switchable and signal-amplified aptasensor based on metal organic frameworks as the quencher for turn-on detection of T-2 mycotoxin. Anal Bioanal Chem 413, 6595–6603 (2021). https://doi.org/10.1007/s00216-021-03625-9

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