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One-step time-resolved fluorescence microsphere immunochromatographic test strip for quantitative and simultaneous detection of DON and ZEN

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Abstract

Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that contaminate a wide range of grains and crops. In this study, a one-step time-resolved single-channel immunochromatographic test strip based on europium ion polystyrene fluorescence microspheres was first developed for sensitive and quantitative detection of DON and ZEN. The concentration of the artificial antigen and the mass ratio of the monoclonal antibody to fluorescent microspheres for conjugation were optimized to simplify the sample addition process during immunochromatographic assay and improve the on-site detection efficiency. The limits of detection (LOD) of the single-channel immunochromatographic test strip for DON and ZEN detection were 0.17 and 0.54 μg/L, respectively. Meanwhile, the dual-channel immunochromatographic test strip was designed to simultaneously detect DON and ZEN, with LODs of 0.24 and 0.69 μg/L achieved for DON and ZEN, respectively. The developed test strips also yielded recovery results consistent with that obtained by LC-MS/MS for DON and ZEN detection in real samples of wheat and corn flour, confirming the practicability and reliability of the test strip. The developed immunochromatographic test strips realize quick and sensitive detection of DON and ZEN, exhibiting potential for broad applications in the point-of-care testing platform of multiple mycotoxins in agricultural products.

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Funding

This research was supported by the National Key Research and Development Program of China (2018YFC1604206), the National Natural Science Foundation of China (No. 31772069), the Natural Science Foundation of Jiangsu Province (No. BK20190584), the fellowship China Postdoctoral Science Foundation (2020M671343), the Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2020-IFST-03), the Collaborative Innovation Center of Food Safety and Quality Control, and the national first-class discipline program of Food Science and Technology (JUFSTR20180303).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu, 214122, People’s Republic of China

    Jiadi Sun, Liangzhe Wang, Diaodiao Yang, Xuran Fu & Xiulan Sun

  2. Comprehensive Technology Center of Zhangjiagang Customs, Zhangjiagang, Jiangsu, 215600, People’s Republic of China

    Jingdong Shao

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  1. Jiadi Sun
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  2. Liangzhe Wang
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  3. Jingdong Shao
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  4. Diaodiao Yang
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  5. Xuran Fu
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  6. Xiulan Sun
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Contributions

Jiadi Sun: investigation, experiment, writing, and review and editing. Liangzhe Wang: data processing and analysis. Diaodiao Yang: data processing. Xuran Fu: data processing, writing. Jingdong Shao: review and editing. Xiulan Sun: visualization, review and editing, supervision.

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Correspondence to Xiulan Sun.

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Sun, J., Wang, L., Shao, J. et al. One-step time-resolved fluorescence microsphere immunochromatographic test strip for quantitative and simultaneous detection of DON and ZEN. Anal Bioanal Chem 413, 6489–6502 (2021). https://doi.org/10.1007/s00216-021-03612-0

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