Abstract
As a novel antioxidant, sodium nitroprusside (SNP) can be used to reduce the adverse effects of various abiotic stresses, especially drought stress. Drought stress is a major problem in the vegetative and reproductive stages of “Mexican lime” (Citrus aurantifolia (Christ.) Swingle), which is known as a main horticultural plant. The aim of the current investigation was to study the impact of SNP on biochemical, morphological, and physiological characteristics of “Mexican lime” under drought stress in vitro condition. This study was performed as a factorial experiment based on a completely randomized design with four replications. Drought stress was induced by using polyethylene glycol (PEG 6000) in four levels (0, 1, 2, and 3%) in Murashige and Skoog (MS) medium. To evaluate the effect of SNP in ameliorating drought stress, various concentrations (0, 25, 50, and 100 μM) of SNP were supplemented to MS medium. The results showed that drought stress led to a reduction in shoot number, shoot length, leaf number, and fresh and dry weight. The application of 25 μM SNP resulted in a favorable impact on these parameters. Also, the highest level of total proline content, electrolyte leakage, and antioxidant enzyme activities were observed under severe drought stress induced by 3% PEG (− 0.3 MPa water potentials).
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The authors would like to thank the College of Agriculture, Shiraz University for their support, cooperation, and assistance throughout this research.
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Marziyeh Jafari: analysis and interpretation of data, methodology, data curation, writing — original draft; Alireza Shahsavar: conceptualization, methodology, project administration, supervision, validation, writing — review and editing.
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Jafari, M., Shahsavar, A.R. Sodium nitroprusside: its beneficial role in drought stress tolerance of “Mexican lime” (Citrus aurantifolia (Christ.) Swingle) under in vitro conditions. In Vitro Cell.Dev.Biol.-Plant 58, 155–168 (2022). https://doi.org/10.1007/s11627-021-10218-9
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DOI: https://doi.org/10.1007/s11627-021-10218-9