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Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment

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Abstract

A novel moderately thermophilic and halophilic bacterium, designated strain M0105T, was isolated from mangrove sediment collected in the Beibu Gulf, south China. The isolate is Gram-negative, non-motile and rod-shaped bacterium with smooth colonies of pale-yellow appearance. Growth occurs at 15–46 °C (optimum 37–40 °C) and pH range of 6.0–10.0 (optimum pH 8.0–9.0). It required 1–7% NaCl (optimum 3–5%) for growth. Strain M0105T was affiliated to the family ‘Rhodobacteraceae’, sharing the highest 16S rRNA gene sequence similarity with Limibaculum halophilum CAU 1123T (96.8%). The major menaquinone Q-10 and the dominant unsaturated fatty acid (C18:1ω7) in this family were also detected in the strain M0105T. The genome sequence possesses a circular 4.1 Mb chromosome with a G + C content of 67.9%. Strain M0105T encoded many genes for cellular stress resistance and nutrient utilization, which could improve its adaptive capacity to the mangrove environment. Values of conserved proteins (POCP), average nucleotide identity, average amino acid identity (AAI) and DNA–DNA hybridization (dDDH) between the isolate and closely related species were below the proposed threshold for species discrimination. Information from phenotypic, chemotaxonomic and phylogenetic analyses proposed that strain M0105T should be assigned to a novel genus within the family ‘Rhodobacteraceae’. Thus, we suggested that the strain M0105T represents a novel species in a new genus, for which the name Thermohalobaculum xanthum gen. nov., sp. nov. is proposed. The type strain of the type species is M0105T (= BGMRC 2019T = KCTC 52118T = MCCC 1K03767T = NBRC 112057T).

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Funding

This study was supported by the Guangxi Natural Science Foundation for Youth Scholar (No. 2018GXNSFBA050021), The Fundamental Research Funds for Guangxi Academy of Sciences (No. 2019YBJ101 and No. 2018YBJ303), Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry (No. 17-259-74), The Special Project for the Base of Guangxi Science and Technology and Talents (Nos. AD17129019 and AD18281066).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, 530007, People’s Republic of China

    Xinli Pan, Zhe Li, Yuanlin Huang, Qiaozhen Wang & Shushi Huang

  2. Guangxi Key Laboratory of Marine Environmental Science, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, 530007, People’s Republic of China

    Fei Li

  3. State Key Laboratory of Non-Food Biomass Energy and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Bioscience and Technology Research Center, Guangxi Academy of Sciences, Nanning, 530007, People’s Republic of China

    Wenjin Hu

  4. Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning, 530008, People’s Republic of China

    Mingguo Jiang

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  1. Xinli Pan
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  2. Zhe Li
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  3. Fei Li
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  4. Yuanlin Huang
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  5. Qiaozhen Wang
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  6. Shushi Huang
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  7. Wenjin Hu
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  8. Mingguo Jiang
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Contributions

ZHL and XP: characterized the bacterium and prepare the manuscript; ZHL, FL, YH, QZH performed the phenotypic and biochemical characterization; SHH helped the analysis of taxonomic data; WH and MJ supervised the study and edited the original draft.

Corresponding authors

Correspondence to Wenjin Hu or Mingguo Jiang.

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The authors declare that there are no conflicts of interest.

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This study does not describe any experimental work related to human.

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The whole genome sequence and the 16S rRNA gene sequence of strain M0105T were deposited in GenBank/EMBL/DDBJ/PIR under Accession Numbers JAEHHL000000000 and MT613384, respectively.

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Pan, X., Li, Z., Li, F. et al. Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment. Antonie van Leeuwenhoek 114, 1819–1828 (2021). https://doi.org/10.1007/s10482-021-01641-4

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  • DOI: https://doi.org/10.1007/s10482-021-01641-4

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