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Colletotrichum species causing anthracnose disease in A. andraeanum, manifested as spathe rot also in addition to spadix rot and leaf spot.

Running title: Colletotrichum spp. causing anthurium anthracnose

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Abstract

Anthurium is an ornamental flowering plant, cultivated in tropical and sub-tropical climates. Anthracnose is a major disease in anthurium, known to be manifested as spadix rot and leaf spot, and caused by Colletotrichum gloeosporioides or its teleomorph, Glomerella cingulata. The objective of the present study was to describe spathe rot, a new disease, and identify Colletotrichum species associated with spathe rot, spadix rot and leaf spot, using multi-locus DNA sequence analyses. Diseased spathes were obtained from anthurium var. Tropical Red while the diseased spadix and leaves were from var. Hawaiian Butterfly. Colletotrichum was isolated from diseased spathe, spadix, and leaf on Potato Dextrose Agar (PDA) following surface sterilization. DNA, extracted from the isolates, was subjected to multigene DNA sequence analyses using ITS, β-tubulin and GAPDH. The two isolates from the spathe rot were identified as C. fructicola and C. orchidearum and the isolates from the spadix rot and leaf spot were identified as C. fruiticola and C. siamense respectively. Koch’s postulates were performed using C. fructicola and C. orchidearum, isolated from the spathe rot, and their pathogenicity was confirmed. The manuscript reports C. fructicola and C. orchidearum as the causal agents of the new disease, the spathe rot, and the association of C. fructicola and C. siamense with the spadix rot and leaf spot respectively.

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Acknowledgements

Authors gratefully acknowledge the University of Peradeniya, Sri Lanka for financial assistance through research grant RG/2014/37/S.

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Authors and Affiliations

  1. Department of Botany, University of Peradeniya, Peradeniya, 20400, Sri Lanka

    I. S. Komala Vithanage, D. M. D. Yakandawala & N. K. B. Adikaram

  2. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 611731, People’s Republic of China

    S. S. N. Maharachchikumbura

  3. National Institute of Fundamental Studies, Hantana Road, Kandy, 20000, Sri Lanka

    L. Jayasinghe & N. K. B. Adikaram

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  1. I. S. Komala Vithanage
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  2. D. M. D. Yakandawala
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  5. N. K. B. Adikaram
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Contributions

All authors contributed to the conception and design of the study. Material preparation, data collection and analysis were performed by [I.S. Komala Vithanage], [D.M.D. Yakandawala], [S.S.N. Maharachchikumbura], [U.L.B. Jayasinghe] and [N.K.B. Adikaram]. The first draft of the manuscript was written by [I.S. Komala Vithanage & N.K.B. Adikaram] and all authors commented on subsequent versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to N. K. B. Adikaram.

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Vithanage, I.S.K., Yakandawala, D.M.D., Maharachchikumbura, S.S.N. et al. Colletotrichum species causing anthracnose disease in A. andraeanum, manifested as spathe rot also in addition to spadix rot and leaf spot.. Eur J Plant Pathol 161, 837–846 (2021). https://doi.org/10.1007/s10658-021-02366-w

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