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Identification of a major QTL related to resistance to soybean mosaic virus in diverse soybean genetic populations

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Abstract

Soybean mosaic virus (SMV) is a member of genus Potyvirus, which causes worldwide soybean [Glycine max (L.) Merr.] yield loss and seed quality deterioration. It is of great significance to find new resistance loci and genes for cultivation of soybean variety. In the present study, a recombinant inbred line (RIL) population and a genome-wide association study (GWAS) panel, which contained 193 lines and 379 germplasms, respectively, were used for QTL mapping of resistance to SMV. Linkage mapping identified a major QTL, qSMV13, on chromosome 13, conferring resistance to SMV SC3 and SC7 strains, explaining phenotypic variations 71.21 and 76.59 %, respectively. The QTL qSMV13 was located close to the known SMV resistance loci Rsv1-h. GWAS analysis revealed five single nucleotide polymorphisms (SNPs) significantly associated with resistance to SC3 on chromosomes 2, 11, 13, 14 and 16. One of the SNP markers, ss715614844, was the right flanking marker of qSMV13. Combining linkage mapping and GWAS analysis enabled us to delimit qSMV13 in a 97.2-kb genomic region containing seven genes. A LRR-RLK protein was proposed as the candidate gene of qSMV13. These results provided selection markers and candidate genes for SMV resistance in soybean molecular breeding programs.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Funding

This study was supported by the Project of Hebei province Science and Technology Support Program (17927670 H, 16227516D-1) and the Natural Science Foundation of Hebei Province, China (C2020204042).

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  1. Jiahao Chu, Wenlong Li contributed equally to this manuscript.

Authors and Affiliations

  1. North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University, Lekai South Street 2596, Baoding City, 071001, Hebei Province, China

    Jiahao Chu, Wenlong Li, Dongri Piao, Xiaobo Huo, Hua Zhang, Hui Du, Youbin Kong, Yuan Jin, Xihuan Li & Caiying Zhang

  2. Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan, 48824, USA

    Feng Lin

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  1. Jiahao Chu
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Contributions

CZ designed the research; JC, WL, DP, XH, HZ, HD, YK, YJ, XL performed experiments; JC, WL and FL analyzed data; JC and WL wrote the manuscript; CZ and XL revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Xihuan Li or Caiying Zhang.

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Chu, J., Li, W., Piao, D. et al. Identification of a major QTL related to resistance to soybean mosaic virus in diverse soybean genetic populations. Euphytica 217, 176 (2021). https://doi.org/10.1007/s10681-021-02907-8

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