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Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood

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Pharmaceutical Chemistry Journal Aims and scope

Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 × 250 mm, 5 μm) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 μg/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.

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Authors and Affiliations

  1. National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan

    M. L. Maheshwari, N. Memon & A. A. Memon

  2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan

    M. L. Maheshwari

  3. Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

    M. Y. Khuhawer

  4. Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan

    A. H. Memon

Authors
  1. M. L. Maheshwari
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  2. N. Memon
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  3. A. A. Memon
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  4. M. Y. Khuhawer
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  5. A. H. Memon
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Correspondence to M. L. Maheshwari.

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Maheshwari, M.L., Memon, N., Memon, A.A. et al. Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood. Pharm Chem J 55, 524–530 (2021). https://doi.org/10.1007/s11094-021-02451-8

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  • DOI: https://doi.org/10.1007/s11094-021-02451-8

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