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A Pilot Study to Determine the Utility of Automated Tissue Dissociator for Flowcytometry Based Evaluation of Hematolymphoid Tumor Tissue Biopsies

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Routine diagnostic biopsy tissue processing, conventional histology/immunohistochemistry (IHC) method is a multi-step and time consuming practice. With the advanced tissue dissociation protocols and panel designing, flow cytometric immunophenotyping (FCI) can be performed on diagnostic hematolymphoid tissue samples using single cell suspensions that economize steps and the time taken. Diagnostic tissue samples from lymph node, mediastinal mass, testicular biopsies and similar sites were dissociated using gentle MACS Octo-dissociator and FCI was performed thereafter. Oral tissue biopsy samples were also processed as a validation set for the protocol. 21 prospective tissue biopsy samples with suspected involvement by a known hematolymphoid neoplasm were processed and evaluated. These included B lymphoblastic lymphoma (n = 12), T lymphoblastic lymphomas (n = 3), Burkitts lymphoma (n = 3) and one case each of granulocytic sarcoma, Hodgkin lymphoma and granulomatous disease. Tissue FCI and IHC were found concordant with identified profile FCI obtained from blood/bone marrow analyses. FCI can produce a highly sensitive and reliable report, within hours, by processing fresh tumor tissue samples from suspected hematolymphoid malignancies. This method can be considered as an effective adjunct to IHC and can be applicable in routine clinical diagnostics, especially in cases that needs quick diagnosis and immediate clinical treatment.

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Acknowledgments

We acknowledge Department of Paediatric Oncology for providing us the tissue samples and Department of Laboratory Hematology and Molecular Genetics for providing us the instrumental support. We also acknowledge Department of Biotechnology (DBT), India for providing the support to carry out the study protocols and experiments under the project SyMeC: GIFT study(EC/GOVT/23/17)26th Feb 2018.

Funding

Department of Biotechnology (DBT), India supported the funding under the project SyMeC (System Medicine Cluster): GIFT (Genome Immune Function of Tumour) study (EC/GOVT/23/17).

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Authors and Affiliations

  1. Department of Laboratory Hematology and Molecular Genetics, Tata Medical Center, Kolkata, 700160, India

    Tanusri Karmakar, Sambhunath Banerjee, Subhajit Brahma, Deepak Kumar Mishra & Neeraj Arora

  2. Department of Histopathology, Tata Medical Center, Kolkata, India

    Debdeep Dey & Geetashree Mukherjee

  3. Department of Clinical Hematology Oncology and HCT, Tata Medical Center, Kolkata, India

    Vivek Radhakrishnan & Mammen Chandy

  4. Department of Pediatric Oncology, Tata Medical Center, Kolkata, India

    Niharendu Ghara & Shekhar Krishnan

Authors
  1. Tanusri Karmakar
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  2. Sambhunath Banerjee
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  3. Subhajit Brahma
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  4. Debdeep Dey
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  5. Vivek Radhakrishnan
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  6. Mammen Chandy
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  7. Niharendu Ghara
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  8. Shekhar Krishnan
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  9. Geetashree Mukherjee
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  10. Deepak Kumar Mishra
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  11. Neeraj Arora
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Contributions

T.K has done the whole work. Both S.B has put their effort on the protocol prepation. DD, VR, MC, NG, SK, GM, DKM and NA has all helped in preparing the manuscript with all necessary suggestions.

Corresponding authors

Correspondence to Tanusri Karmakar or Neeraj Arora.

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Conflict of interest

None of the authors state any conflict of interest with this work.

Human or Animals Participants

This research study involved a group of 21 patient samples predominantly with suspected involvement by a known haematolymphoid neoplasm. The study was accepted through the ethical clearance approved from the Institutional Review Board.

Informed Consent

Informed consent was taken from each patient for being included in the study and will receive standard of care institutional practise for the disease and periodic follow up thereafter.

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Karmakar, T., Banerjee, S., Brahma, S. et al. A Pilot Study to Determine the Utility of Automated Tissue Dissociator for Flowcytometry Based Evaluation of Hematolymphoid Tumor Tissue Biopsies. Indian J Hematol Blood Transfus 38, 403–410 (2022). https://doi.org/10.1007/s12288-021-01481-2

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  • DOI: https://doi.org/10.1007/s12288-021-01481-2

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