Abstract
In the context of peripheral nerve injuries treatment, self-electrical stimuli nerve guidance conduit is a promising technique. To fabricate such structures, PVDF-chitosan–gelatin was considered for the outside walls of conduit and gellan gum containing conductive polyaniline-graphene (PAG) nanocomposite particles in the middle. PVDF-chitosan–gelatin nanofibers were prepared using the dual-electrospinning method and highly conductive binary-doped polyaniline-graphene was synthesized by chemical oxidative polymerization in the presence of aniline and sodium dodecyl sulfate. The morphology and chemical structure of nanofibers and PAG were characterized using SEM and FTIR analyses. The morphological, structural, electrical, and mechanical properties of gellan containing PAG particles were investigated with SEM, FTIR, piezoelectric and rheology tests. Gelation time, swelling, and degradation of gellan PAG were also studied. Morphological investigation of self-electrical stimuli conduit represents successful electrospinning and the polymerization of polyaniline was confirmed using FTIR and XRD methods. The designed conduit shows the adequate output voltage and highly oriented pore structures in gellan gel as an in-situ thermosensitive construction makes convenient properties for peripheral nerve injury treatment. Our obtained results illustrate that the self-electrical nerve guidance conduit with gellan PAG can provide a novel substrate as a neural conduit.
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Mohseni, M., Ramazani Saadatabadi, A. Highly conductive self-electrical stimuli core-shell conduit based on PVDF-chitosan–gelatin filled with in-situ gellan gum as a possible candidate for nerve regeneration: a rheological, electrical, and structural study. Appl Nanosci 11, 2199–2213 (2021). https://doi.org/10.1007/s13204-021-02012-1
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DOI: https://doi.org/10.1007/s13204-021-02012-1