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Factors Determining Barrier Properties to Oxygen Transport Across Model and Cell Plasma Membranes Based on EPR Spin-Label Oximetry

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Abstract

This review is motivated by the exciting new area of radiation therapy using a phenomenon termed FLASH in which oxygen is thought to have a central role. Well-established principles of radiation biology and physics suggest that if oxygen has a strong role, it should be the level at the DNA. The key aspect discussed is the rate of oxygen diffusion. If oxygen freely diffuses into cells and rapidly equilibrates, then measurements in the extracellular compartment would enable FLASH to be investigated using existing methodologies that can readily measure oxygen in the extracellular compartment. EPR spin-label oximetry allows evaluation of the oxygen permeability coefficient across lipid bilayer membranes. It is established that simple fluid-phase lipid bilayers are not barriers to oxygen transport. However, further investigations indicate that many physical and chemical (compositional) factor can significantly decrease this permeation. In biological cell plasma membranes, the lipid bilayer forms the matrix in which integral membrane proteins are immersed, changing organization and properties of the lipid matrix. To evaluate oxygen permeability coefficients across these complex membranes, oxygen permeation across all membrane domains and components must be considered. In this review, we consider many of the factors that affect (decrease) oxygen permeation across cell plasma membranes. Finally, we address the question, can the plasma membrane of the cell form a barrier to the free diffusion of oxygen into the cell interior? If there is a barrier then this must be considered in the investigations of the role of oxygen in FLASH.

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reproduced from Fig. 4 of “Changes in the Properties and Organization of Human Lens Lipid Membranes Occurring with Age” by L. Mainali, M. Raguz, W. J. O’Brien, and W. K. Subczynski, in Current Eye Research, first published online 28 Oct 2016, reprinted by permission of the publisher (Taylor & Francis Ltd, http://www.tandfonline.com). Copyright 2016

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Research reported in this publication was supported by the National Eye Institute of the National Institutes of Health under award number R01 EY015526. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA

    Witold K. Subczynski & Natalia Stein

  2. Department of Biophysics, Medical University of Lublin, Jaczewskiego 4, Lublin, Poland

    Justyna Widomska

  3. Department of Radiology, The Geisel School of Medicine At Dartmouth, Lebanon, NH, 03766, USA

    Harold M. Swartz

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  1. Witold K. Subczynski
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Subczynski, W.K., Widomska, J., Stein, N. et al. Factors Determining Barrier Properties to Oxygen Transport Across Model and Cell Plasma Membranes Based on EPR Spin-Label Oximetry. Appl Magn Reson 52, 1237–1260 (2021). https://doi.org/10.1007/s00723-021-01412-4

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