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Evaluation of potent cyanobacteria species for UV-protecting compound synthesis using bicarbonate-based culture system

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Abstract

The present investigation evaluates the potential of three cyanobacteria species Anabaena cylindrica, Nostoc commune and Synechococcus BDUSM-13 for photo-protecting mycosporine-like amino acids (MAAs) synthesis using bicarbonate-based culture system. Current investigations witnessed noteworthy bicarbonate tolerance of all species (NaHCO3; 0.5, 1 and 2 g L− 1) in terms of their growth rate, chlorophyll content, biomass productivity and carbon fixation ability. Among all strains, Synechococcus BDUSM-13 showed maximum surge in specific growth rate (i.e. 0.72 day−1) at 1 g L−1, productivity (i.e. 0.92 ± 0.06 g day−1 L−1) and chlorophyll content (i.e. 0.09 g L−1) at 2 g day−1 L−1. Synechococcus cells were also has the 0.48 g dw−1 carbon content with highest CO2 fixation rate (i.e. 0.653 g.CO2 mL−1 day−1) at 2 g L−1. Though, they were not able to produce MAAs after long UV-B exposure (i.e. 24 and 48 h). A. cylindrica strain was the most competent species for the bicarbonate-based approach, produced UV-protecting iminomycosporine compound (i.e. shinorine, λmax at 334 ± 2 nm) along with carbon fixation (i.e. 0.49 g CO2 mL−1 day−1) at 2 g L−1 NaHCO3. This suggests the bicarbonate supplementation during cultivation is a promising strategy to increase cellular abundance, biomass productivity and carbon fixation in cyanobacteria. However, UV-B irradiation may cause species-specific differences in the MAAs synthesis to produce UV-protecting compounds.

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Acknowledgements

Authors are thankful to the University of Allahabad, Prayagraj, India for providing necessary laboratory facilities. Dr. Shailendra Kumar Singh gratefully acknowledges the financial support from the University Grants Commission (UGC) through Dr. D. S. Kothari Postdoctoral Scheme. Rupali Kaur is also thankful to the UGC for providing D. Phil. fellowship for the financial assistance.

Funding

This work was supported by the University Grants Commission, New Delhi, India [Grant number: EN/19-20/0020].

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Authors and Affiliations

  1. Centre of Biotechnology, Nehru Science Centre, University of Allahabad, Prayagraj, 211002, India

    Shailendra Kumar Singh, Rupali Kaur, Manjita Mishra & Shanthy Sundaram

  2. Department of Biotechnology, S. S. Khanna Girls Degree College, Prayagraj, 211012, India

    Md. Akhlaqur Rahman

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  1. Shailendra Kumar Singh
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Contributions

All authors provided critical feedback and helped shape the research, analysis and manuscript. SKS, RK, MAR, MM and SS are the authors of manuscript entitled “An integrated approach to produce UV-protecting compounds in cyanobacterial strains coupled with CO2 sequestration”. Prof. SS and Dr. SKS conceived of the presented idea and encouraged RK and MM investigated the proposed approach and carried out the experiment. Dr. MAR verified the analytical methods. Dr. SKS wrote the manuscript with support from MAR, and SS. The overall project has been completed under the mentorship of corresponding author Prof. SS.

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Correspondence to Shanthy Sundaram.

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Singh, S.K., Kaur, R., Rahman, M.A. et al. Evaluation of potent cyanobacteria species for UV-protecting compound synthesis using bicarbonate-based culture system. 3 Biotech 11, 412 (2021). https://doi.org/10.1007/s13205-021-02945-y

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