Abstract
Lignin is essential for the characteristics and quality of timber. Nitrogen has significant effects on lignin contents in plants. Nitrogen has been found to affect wood quality in plantations and lignin content in plants. Caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) is an important methyltransferase in lignin biosynthesis. However, the classification of woody plant CCoAOMT gene family members and the regulation mechanism of nitrogen are not clear. Bioinformatics methods were used to predict the members, classification, and transcriptional distribution of the CCoAOMT gene family in Populus trichocarpa. The results showed that there were five PtCCoAOMTs identified, and they could be divided into three sub-groups according to their structural and phylogenetic features. The results of tissue expression specificity analysis showed that: PtCCoAOMT1 was highly expressed in roots and internodes; PtCCoAOMT2 was highly expressed in roots, nodes, and internodes, PtCCoAOMT3 was highly expressed in stems; PtCCoAOMT4 was highly expressed in young leaves, and, PtCCoAOMT5 was highly expressed in roots. Different forms and concentrations of nitrogen had varying effects on the expression patterns of genes in different plant tissue types. The results of real-time PCR showed that the expression levels of PtCCoAOMT1 and PtCCoAOMT2 in stems increased significantly under different forms of nitrogen. PtCCoAOMT3 and PtCCoAOMT4 were induced by nitrate nitrogen in upper stems and lower leaves, respectively. PtCCoAOMT4 and PtCCoAOMT5 were induced by different concentrations of nitrate nitrogen in lower stems and roots, respectively. These results could provide valuable information for revealing the differences between functions and expression patterns of the various CCoAOMT gene family members under different forms and concentrations of exogenous nitrogen in poplar.
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Acknowledgements
This work was supported by “the Fundamental Research Funds for the Central Universities (Grant No. 2572020BA02)”, “the National Natural Science Foundation of China (Grant Nos. 31570648, 31600534)”, “Natural Science Foundation of Heilongjiang Province, China (Grant No. c2018009)”, “The Fundamental Research Funds for the Central Universities (Grant No. 2572019CT02)” and “The 111 Project (Grant No. B16010)”.
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HZ and CQ conceived and designed the study, HZ and CQ performed most of the experiments. SZ and LC conducted the sampling, ZZ and XX performed bioinformatics calculations. GL and ZX processed and analyzed the data, and HZ, CQ, and GL wrote the manuscript.
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Supplementary file1 (TIF 3373 KB)
Figure S1 Expression of PtCCoAOMT gene family members under different nitrogen forms and concentrations.
Supplementary file2 (TIF 1065 KB)
Figure S2 Multiple alignment of the suspected gene with five PtCCoAOMT amino acid sequences.
Supplementary file3 (TIF 1640 KB)
Figure S3 Analysis of the gene exon-intron structure in the suspected gene.
Supplementary file4 (TIF 39 KB)
Figure S4 Tissue-specific expression profiles of suspected gene.
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Zhao, H., Qu, C., Zuo, Z. et al. Genome Identification and Expression Profiles in Response to Nitrogen Treatment Analysis of the Class I CCoAOMT Gene Family in Populus. Biochem Genet 60, 656–675 (2022). https://doi.org/10.1007/s10528-021-10112-4
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DOI: https://doi.org/10.1007/s10528-021-10112-4