Abstract
Fe(III) reducing bacteria (FeRB) play a vital role in the biogeochemical cycle of Fe, C and N in nature. The volcanic lake can be considered as an ideal habitat for FeRB. Here, we investigated the diversity and spatial distribution of FeRB in sediments of Wenbo lake in Wudalianchi volcano based on culture-dependent and independent methods. A total of 28 isolates affiliated with the genera of Enterobacter, Bacillus, Pseudomonas and Clostridium were obtained from 18 sediment samples. We detected 783 operational taxonomic units (OTUs) belonged to FeRB using high high-throughput sequencing, and the dominant phyla were Proteobacteria (3.65%), Acidobacteria (0.29%), Firmicutes (10.78%). The representative FeRB genera such as Geobacter, Pseudomonas, Thiobacillus and Acinetobacter distributed widely in Wenbo lake. Results showed that the diversity and abundance of FeRB declined along the water-flow direction from Libo to Jingbo. In contrast, the FeRB diversity decreased and the FeRB abundance increased along with depth transect of sediments. It was found that the dominant phylum changed from Firmicutes to Proteobacteria along the water-flow direction, while changed from Proteobacteria to Firmicutes along with the depth of sediments. RDA indicated that the FeRB distribution were driven by soluble total iron, total organic carbon, Fe(II) and Fe(III). These will provide information for understanding the role of FeRB in the elements geochemical cycles in the volcanic environment.
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Data availability
Accession numbers for FeRB isolates are MK522128-MK522146, MK530142-MK530146, MK478917-MK478918, and MK910758. The raw sequence reads were deposited in the Short Reads Archive (SRA) database and were publicly available with the accession number SAMN13091624-SAMN13091659.
Abbreviations
- FeRB:
-
Fe(III) reducing bacteria
- STI:
-
Soluble total iron
- TOC:
-
Total organic carbon
- FeOB:
-
Fe(II) oxidizing bacteria
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (41471201), the Longjiang Scholar Program of Heilongjiang Province (Q201815), the Natural Science Foundation of Heilongjiang Province of China (LH2020C079), the Talent Training Program under Special Funds Supporting the Development of Local Universities from the Central Finance (HFBE[2019]465), the Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong (TDJH201809), the National Key Research and Development Program of China (2018YFD0800906), the Technology Program of Land Reclamation General Bureau of Heilongjiang (HKKY190406), and the Program of Science and Technology Innovation Team of the Heilongjiang Higher Education Institutions (2012TD006).
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Zhan, Y., Yang, M., Zhang, Y. et al. Iron and total organic carbon shape the spatial distribution pattern of sediment Fe(III) reducing bacteria in a volcanic lake, NE China. World J Microbiol Biotechnol 37, 155 (2021). https://doi.org/10.1007/s11274-021-03125-z
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DOI: https://doi.org/10.1007/s11274-021-03125-z