Skip to main content
SpringerLink
Log in

Comparative efficacy of Ovaprim and hMG (menotropin) to induce breeding in African catfish (Clarias gariepinus)

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The applications of exogenous hormones in different species for the induction of oocyte production, final oocyte maturation (FOM), and spawning for their reproduction is getting more attention day by day. The current preliminary research work was carried out to induce breeding in Clarias gariepinus, commonly known as African catfish, imported from Thailand. Single doses of two hormones as Ovaprim and human menopausal gonadotropin (hMG) were used and the research work was carried out at Muzaffargarh Fish Hatchery Punjab, Pakistan. A total of twenty-four (n = 24) C. gariepinus were selected having body weight approximately 2 kg and divided into two main groups based on gender as male (n = 12) and female (n = 12). For milt collection, all males were treated with Ovaprim 0.5 mg/kg body weight (b.w.) and female fish were divided into three groups as A, B, and C with four (n = 4) fish in each group. Group A was injected with only normal saline (control group) while fish in group B and group C were treated with hMG at 0.5 mg/kg b.w. and Ovaprim 0.5 mg/kg b.w., respectively. Then, after 6 h of hormone injections until 48 h, spawned eggs, eggs’ weight, fertilization rate, hatching rate, survival rate, fecundity, and deformed larvae were investigated. The results revealed that Ovaprim injection significantly (p < 0.05) modulate the reproductive parameters in group C while no breeding was induced in both control and hMG-treated groups. Hence, it could be concluded that Ovaprim has the potential to induce breeding in African catfish, while in the current study, hMG failed to induce breeding. However, trials at large scales are required to further explore the effect of different doses of both tested hormones by increasing the treated subjects particularly in Pakistani fish farms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Data availability

Data will be provided on request.

Code availability

Not applicable.

References

  • Achionye–nzeh C, Obaroh I (2012) Ovaprim doses effects on eggs of African mudfish Clarias gariepinus. Int J Life Sci Pharm Res 2(1):6–9

    Google Scholar 

  • Adebayo O, Popoola O (2008) Comparative evaluation of efficacy and cost of synthetic and non-synthetic hormones for artificial breeding of African catfish (Clarias gariepinus Burchell, 1822). J Fish Aquat Sci 3(1):66–71

    Article  Google Scholar 

  • Ali MA, Rasheed SB, Hassan Z, Ibrar M, Majeed A, Ulhaq Z et al (2015) Efficacy of synthetic hormones Ovatide and Ovaprim in induced breeding of major Indian and Chinese carps. J Agric Technol 11(7):1449–1456

    CAS  Google Scholar 

  • Basu D, Rana G, Mondal B, Sengupta K, Dhar P (2000) Studies on the comparative efficacy of Ovaprim, HCG and piscine pituitary gland in induced breeding of Clarias batrachus,(Linn). Fishing Chimes 19(10–11):103–104

    Google Scholar 

  • Bruton MN (1979) The breeding biology and early development of Clarias gariepinus (Pisces: Clariidae) in Lake Sibaya, South Africa, with a review of breeding in species of the subgenus Clarias (Clarias). Trans Zool Soc London 35(1):1–45

    Article  Google Scholar 

  • Brzuska E, Adamek J (1999) Artificial spawning of European catfish, Silurus glanis L.: stimulation of ovulation using LHRH‐a, Ovaprim and carp pituitary extract. Aquacult Res 30(1):59–64

    Article  Google Scholar 

  • Brzuska E (2004) Artificial propagation of African catfish (Clarias gariepinus): the application of a single dose of pellets containing D-Ala6, Pro9NEt-mGnRH and dopamine inhibitor metoclopramide. Czech J Anim Sci UZPI (Czech Republic). pp 289–296

  • Cheah M, Lee C (2000) Induced ovulation of the Australian eel-tailed catfish Neosilurus ater (Perugia) with Ovaprim. Asian Fish Sci 13(1):87–96

    Google Scholar 

  • Dhawan A, Kaur K (2004) Comparative efficacy of Ovaprim and Ovatide in carp breeding. Indian J Fish 51(2):227–228

    Google Scholar 

  • DiMaggio MA, Broach JS, Ohs CL (2013) Evaluation of Ovaprim and human chorionic gonadotropin doses on spawning induction and egg and larval quality of pinfish. Lagodon Rhomboides Aquacult 414:9–18

    Article  Google Scholar 

  • Francis T, Ramanathan N, Athithan S, Cheryl H (2000) Induced breeding of Murrel, Channa striatus using various inducing agents. Fishing Chimes 19(10):191–221

    Google Scholar 

  • Haniffa M, Nagarajan M, Marimuthu K, Raj AA (2003) Embryonic and larval development of spotted murrel, Channa punctatus (Bloch). Indian J Fish 50(3):355–362

    Google Scholar 

  • Haylor G (1991) Controlled hatchery production of Clarias gariepinus (Burchell 1822): growth and survival of fry at high stocking density. Aquac Res 22(4):405–422

    Article  Google Scholar 

  • Henk JT, Richter CJ (1996) Internal and external factors controlling reproduction in the African catfish Clarias Gariepinus. Aquat Living Resour 9(S1):45–58

    Google Scholar 

  • Henken A, Brunink A, Richter C (1987) Differences in growth rate and feed utilization between diploid and triploid African catfish, Clarias gariepinus (Burchell 1822). Aquaculture 63(1–4):233–242

    Article  Google Scholar 

  • Hogendoorn H, Vismans M (1980) Controlled propagation of the African catfish, Clarias lazera (C. & V.): II. Artificial reproduction. Aquaculture 21(1):39–53

    Article  Google Scholar 

  • Kasi M, Nirmell S, Aminur Rahman M, Arshad A, Gokul Raj M, Arockiaraj J (2015) Induced ovulation and spawning of African catfish Clarias gariepinus (Bloch) using Ovaprim. J Environ Biotechnol Res 1(1):2–9

    Google Scholar 

  • Mahapatra B, Sengupta K, De U, Rana G, Datta A, Basu A et al (2000) Controlled breeding and larval rearing of Clarias batrachus (Linn.) for mass scale propagation. Fishing Chimes 19(10 & 11):97–102

    Google Scholar 

  • Maradun HF, Umar F, Ibrahim A, Mubarak A, Zarau IJ, Muhammad SA (2018) Effect of different doses of Ovulin hormone on the induced breeding performance of Clarias. J Anim Vet Sci 5(1):1–5

    Google Scholar 

  • Marimuthu K, Haniffa MA, Rahman MA (2009) Spawning performance of native threatened spotted snakehead fish, Channa punctatus (Actinopterygii: Channidae: Perciformes), induced with Ovatide. Acta Ichthyol Piscat 39(1):1

    Article  Google Scholar 

  • Marimuthu K, Satthiyasilan N, Amin ur Rahman M, Arshad A, Gokul R M, Arockiaraj J (2015) Induced ovulation and spawning of African catfish Clarias gariepinus using ovaprim. Environ Biotechnol 1(1):2–9

  • Nandeesha MC, Rao KG, Jayanna R, Parker NC, Varghese TJ, Keshavanath P et al. editors (1990) Induced spawning of Indian major carps through single application of Ovaprim. the second Asian fisheries forum (Eds: R Hirano and M Hanyu) Asian Fisheries Society, Manila, Philippines pp 581–585

  • Olubiyi O, Ayinia O, Adeyemo A (2005) The effects of various doses of Ovaprim on reproductive performance of the African catfish Clarias gariepinus (Burchell) and Heterobranchus longifilis (Valenciennes). Afr J Appl Zool Environ Biol 7(1):101–105

    Google Scholar 

  • Peter R, Yu K (1997) Neuroendocrine regulation of ovulation in fishes: basic and applied aspects. Rev Fish Biol Fish 7(2):173–197

    Article  Google Scholar 

  • Richter C, Eding E, Goos HT, De Leeuw R, Scott A, Van Oordt P (1987) The effect of pimozide/LHRHa and 17α-hydroxyprogesterone on plasma steroid levels and ovulation in the African catfish, Clarias gariepinus. Aquaculture 63(1–4):157–168

    Article  CAS  Google Scholar 

  • Sahoo S, Giri S, Chandra S (2008) Induced spawning of Clarias batrachus (Linn): effect of Ovaprim doses and latency periods on the weight of stripped eggs and ovary. Asian Fish Sci 21:333–338

    Google Scholar 

  • Sahoo S, Giri S, Sahu A (2005) Effect on breeding performance and egg quality of Clarias batrachus (Linn.) at various doses of Ovatide during spawning induction. Asian Fish Sci 18(1/2):77

    Google Scholar 

  • Shepherd CJ, Bromage NR (1988) Intensive fish farming: Blackwell Scientific Publications Ltd p 201–222

  • Shinkafi B, Ilesanmi B (2014) Effect of varying doses of Ovatide on the breeding performance of African catfish (Clarias gariepinus Burchell, 1822) in Sokoto, North-western Nigeria. Asian J Anim Sci 8(2):56–64

    Article  Google Scholar 

  • Sridhar S, Vijayakumar C, Haniffa M (1998) Induced spawning and establishment of a captive population for an endangered fish Ompok bimaculatus in India. Curr Sci 75(10):1066–1068

    Google Scholar 

  • Uys W (1989) Aspects of the nutritional physiology and dietary requirements of juvenile and adult sharptooth catfish, Clarias gariepinus (Pisces: Clariidae): Rhodes University Grahamstown, South Africa pp 33–97

  • Vijaykumar S, Sridhar S, Haniffa M (1998) Low cost breeding and hatching techniques for the catfish (Heteropneustes fossilis) for small-scale farmers Naga. ICLARM Q 21(4):15–17

    Google Scholar 

  • Viveen W, Richter C, Van Oordt P, Janssen J, Huisman E (1986) Practical manual for the culture of the African catfish (Clarias gariepinus). 15–94

  • Zohar Y, Mylonas CC (2001) Endocrine manipulations of spawning in cultured fish: from hormones to genes. Reproductive Biotechnology in Finfish Aquaculture: Elsevier p 99–136

Download references

Author information

Authors and Affiliations

  1. Department of Fisheries, Government of Punjab, Rajanpur, 33500, Pakistan

    Muhammad Wajahat Ameer

  2. Department of Zoology, Faculty of Life Sciences, Government College University Faisalabad, Faisalabad, Pakistan

    Farhat Jabeen, Muhammad Asad, Ghazala Kaukab, Amnah Bashir, Misha Rasheed, Hafsa Younis & Javaria Nawaz

  3. Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan

    Naveed Munir

  4. Department of Eastern Medicine, Government College University Faisalabad, Faisalabad, Pakistan

    Rida Zainab & Muhammad Akram

Authors
  1. Muhammad Wajahat Ameer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Farhat Jabeen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Muhammad Asad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ghazala Kaukab
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Amnah Bashir
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Misha Rasheed
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hafsa Younis
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Naveed Munir
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Javaria Nawaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Rida Zainab
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Muhammad Akram
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed equally.

Corresponding authors

Correspondence to Farhat Jabeen or Muhammad Akram.

Ethics declarations

Ethics approval

Ethical approval for this research was taken from the Ethical Research Board for Animal Government College University Faisalabad (GCUF-DZ-2018).

Consent to participate

Not applicable

Consent for publication

Not applicable

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ameer, M.W., Jabeen, F., Asad, M. et al. Comparative efficacy of Ovaprim and hMG (menotropin) to induce breeding in African catfish (Clarias gariepinus). Fish Physiol Biochem 47, 1559–1564 (2021). https://doi.org/10.1007/s10695-021-01003-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-021-01003-x

Keywords

Search

Navigation