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Zinc and l-Ascorbic Acid Dietary Supplementation Affected Antioxidant Status, Heat Shock Protein 70, and Some Immunoglobulins in Japanese Quail Under Heat Stress Conditions

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Abstract

This study assessed the impact of the dietary inclusion of l-ascorbic acid and organic zinc (Availa-Zn) on heat-stressed Japanese quails. Growth performance, antioxidant status, immune status, heat shock protein 70 (HSP70), and some blood biochemical parameters were assessed. One-day-old, unsexed Japanese quail chicks (n = 240) were randomly allocated into 4 dietary treatments (6 replicates per treatment; 10 birds per replicate). Birds were fed a basal corn-soybean meal diet (control treatment) with different supplemental levels of l-ascorbic acid and/or Availa-Zn (200 mg l-ascorbic acid/kg diet, 62 mg Availa-Zn/kg diet, and 200 mg l-ascorbic acid + 62 mg Availa-Zn/kg diet) from July to August 2020 for 35 days. The average minimum and maximum ambient temperatures varied from 85.4 to 98.8 °F, and the relative humidity was between 69 and 74%. Supplemented l-ascorbic acid and Availa-Zn, either as separate supplements or as combined supplements, increased bird growth performance, blood hemoglobin, thyroid hormones, total protein, globulin, total antioxidant capacity, HSP70, catalase, superoxide dismutase enzyme activity, and immunoglobulin A and G (P < 0.05), while heterophil/lymphocytes decreased (P < 0.01) during the entire rearing period (1–35 days). Most of the assessed parameters showed stronger responses when l-ascorbic acid and Availa-Zn were added together, which may suggest a synergistic effect. In conclusion, the combined supplementation of l-ascorbic acid and Availa-Zn at 200 and 62 mg/kg, respectively, could be considered an efficient dietary supplement to enhance Japanese quail growth performance, antioxidant capacity, immune status, and general health under heat stress conditions.

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Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors would like to thank their respected universities and institutes.

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Authors and Affiliations

  1. Animal Production Deptartment, Agricultural and Biological Research Division, National Research Center, Dokki, Cairo, 11865, Egypt

    Ahmed Gouda & Mosaad M. El-Moniary

  2. Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

    Samar A. Tolba

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  1. Ahmed Gouda
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  2. Samar A. Tolba
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  3. Mosaad M. El-Moniary
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Contributions

A. G., M. M. E., and S. A. T. designed the study plan. A. G. and M. M. E. conducted the research work and collected the data. S. A. T. analyzed the data and drafted the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Samar A. Tolba.

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Ethics Statement

The ethical approval was obtained from the Institutional Animal Care and Use Committee of Zagazig University, Egypt (Approval No. ZU-IACUC/2/F/115/2020).

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The authors declare no competing interests.

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Gouda, A., Tolba, S.A. & El-Moniary, M.M. Zinc and l-Ascorbic Acid Dietary Supplementation Affected Antioxidant Status, Heat Shock Protein 70, and Some Immunoglobulins in Japanese Quail Under Heat Stress Conditions. Biol Trace Elem Res 200, 2889–2896 (2022). https://doi.org/10.1007/s12011-021-02884-y

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  • DOI: https://doi.org/10.1007/s12011-021-02884-y

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