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Biosynthesis of the nucleoside antibiotic angustmycins: identification and characterization of the biosynthetic gene cluster reveal unprecedented dehydratase required for exo-glycal formation

The Journal of Antibiotics volume 74, pages 830–833 (2021)Cite this article

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Abstract

The nucleoside antibiotic angustmycin, produced by some Streptomyces strains, is composed of adenine and C6 sugar and shows antibiotic and antitumor activities. In this study, we propose a biosynthetic pathway for angustmycin using a heterologous expression experiment coupled with in silico analysis of the angustmycin biosynthetic gene (agm) cluster. The biochemical characterization of Agm6 demonstrated its role in angustmycin biosynthesis as an unprecedented dehydratase.

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Acknowledgements

This work was supported by JSPS KAKENHI (21H02127) and JSPS A3 Foresight Program (16822333) to T. Kuzuyama.

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  1. Taro Shiraishi

    Present address: Eidgençssische Technische Hochschule (ETH) Zürich, Institute of Microbiology, Zurich, Switzerland

Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Taro Shiraishi, Jiaqi Xia, Teruhito Kato & Tomohisa Kuzuyama

  2. Collaborative Research Institute for Innovative Microbiology (CRIIM), The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Tomohisa Kuzuyama

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  1. Taro Shiraishi
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Correspondence to Tomohisa Kuzuyama.

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Shiraishi, T., Xia, J., Kato, T. et al. Biosynthesis of the nucleoside antibiotic angustmycins: identification and characterization of the biosynthetic gene cluster reveal unprecedented dehydratase required for exo-glycal formation. J Antibiot 74, 830–833 (2021). https://doi.org/10.1038/s41429-021-00466-7

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