Abstract
A bacterial strain, designated M625T, was isolated from the surface of a marine red alga. Phylogenetic trees were reconstructed based on the 16S rRNA gene and RpoB protein sequences, which indicated that the strain belongs to the genus Aquimarina within the family Flavobacteriaceae. Strain M625T showed high sequence similarities to A. aggregata RZW4-3-2 T (95.7%), A. seongsanensis CBA3208T (95.3%) and A. versatilis CBA3207T (95.0%). The AAI and POCP values between strain M625T and A. muelleri DSM 19832 T were 71.8% and 57.9% respectively. The dDDH and ANI values between strain M625T and A. aggregata were 19.5% and 74.6% respectively. The strain was Gram-stain negative, strictly aerobic, non-motile and long rod-shaped, and positive for hydrolysis of starch, cellulose, alginate, DNA and Tween 20. The dominant respiratory quinone was MK-6. The major fatty acids were iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G, and the polar lipids consisted of phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids, and seven unidentified lipids. Based on the polyphasic comparisons, strain M625T is proposed to represent a novel species within the genus Aquimarina, for which the name Aquimarina algicola sp. nov. (type strain M625T = MCCC 1H00399T = KCTC 72685 T) was proposed.
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Acknowledgements
This work of scanning electron microscope was supported by Physical-Chemical Materials Analytical & Testing Center of Shandong University at Weihai.
Funding
This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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XKS analyzed most of the data and wrote the manuscript. YLZ contributed to providing critical revisions to this article. XYC was responsible for collecting samples and isolating the novel microorganism. ZJD and GJC designed all the experiments and supervised the manuscript.
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The GenBank accession number for the 16S rRNA gene sequence of Aquimarina algicola M625T is MN822655, and the draft genome has been deposited in GenBank under the accession number VFWZ00000000.
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Sun, XK., Zhong, YL., Chen, XY. et al. Aquimarina algicola sp. nov., isolated from the surface of a marine red alga. Arch Microbiol 203, 5397–5403 (2021). https://doi.org/10.1007/s00203-021-02524-y
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DOI: https://doi.org/10.1007/s00203-021-02524-y