Abstract
A Gram-positive, acid-fast and rapidly growing rod, designated S2-37 T, that could form yellowish colonies was isolated from one soil sample collected from cotton cropping field located in the Xinjiang region of China. Genomic analyses indicated that strain S2-37 T harbored T7SS secretion system and was very likely able to produce mycolic acid, which were typical features of pathogenetic mycobacterial species. 16S rRNA-directed phylogenetic analysis referred that strain S2-37 T was closely related to bacterial species belonging to the genus Mycolicibacterium, which was further confirmed by pan-genome phylogenetic analysis. Digital DNA-DNA hybridization and the average nucleotide identity presented that strain S2-37 T displayed the highest values of 39.1% (35.7–42.6%) and 81.28% with M. litorale CGMCC 4.5724 T, respectively. And characterization of conserved molecular signatures further supported the taxonomic position of strain S2-37 T belonging to the genus Mycolicibacterium. The main fatty acids were identified as C16:0, C18:0, C20:3ω3 and C22:6ω3. In addition, polar lipids profile was mainly composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Phylogenetic analyses, distinct fatty aids and antimicrobial resistance profiles indicated that strain S2-37 T represented genetically and phenotypically distinct from its closest phylogenetic neighbour, M. litorale CGMCC 4.5724 T. Here, we propose a novel species of the genus Mycolicibacterium: Mycolicibacterium gossypii sp. nov. with the type strain S2-37 T (= JCM 34327 T = CGMCC 1.18817 T).
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Acknowledgements
The authors thank Dr. Zhi-Dong Zhang from Xinjiang Academy of Agricultural Sciences of China for his assistance with sampling.
Funding
This work was supported by grants from the Natural Science Foundation of Jiangsu Province (Grant No. BK20190703) and The Natural Science Research of the Jiangsu Higher Education Institutions of China (Grant No. 19KJB530010).
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RRH performed the experiments and finished the draft of the manuscript. RRH, SRY, CZ, XFG and XKC performed strain isolation and phenotypic analyses. RRH and SRY performed genomic analyses. ZQW and YNL assisted to improve the manuscript. WZL designed all the experiments and supervised the manuscript.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain S2-37 T is MW295419. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAFEVR000000000. The version described in this paper is version JAFEVR010000000.
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Huang, RR., Yang, SR., Zhen, C. et al. Genomic molecular signatures determined characterization of Mycolicibacterium gossypii sp. nov., a fast-growing mycobacterial species isolated from cotton field soil. Antonie van Leeuwenhoek 114, 1735–1744 (2021). https://doi.org/10.1007/s10482-021-01638-z
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DOI: https://doi.org/10.1007/s10482-021-01638-z