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Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea

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Abstract

A bacterial lipase producing bacterium, designated SCS 2–3, was isolated from deep-sea sediment of the South China Sea. Phylogenetic analysis based on the 16S rRNA sequence revealed that strain SCS2-3 belonged to the genus Pseudomonas and had 98.56% similarity to P. xinjiangensis NRRL B-51270T as the closest relative strain. MLSA using four protein-coding genes (dnaK, gyrA, recA, and rpoB) showed strain SCS 2–3 to form a separate branch. ANI and in silico DDH values between strain SCS 2–3 and related type strains of Pseudomonas were less than 81.51% and 23.80%, respectively. Genome comparison showed that strain SCS 2–3 shared 1875 core gene families with other eight closely related type strains in Pseudomonas, and the number of strain-unique genes was 263. Through gene annotations, genes related to lipase were found in the genome. Furthermore, a combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SCS 2–3 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nanhaiensis sp. nov. is proposed. The type strain is SCS 2–3T (= GDMCC 1.2219T = JCM 34440T).

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Funding

This work was supported by National Key R&D Program of China (No.2018YFA0901000, 2018YFA0901003), the National Transgenic Major Program of China (No. 2019ZX08010-004), the National Natural Science Foundation of China (No. 31930004). We also appreciate the support of the Agricultural Science and Technology Innovation Program of CAAS (No. CAAS-ZDRW202009).

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Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yu Pang, Yage Zhang, Mengru Chen, Wei Lu, Ming Chen, Yongliang Yan, Min Lin, Wei Zhang & Zhengfu Zhou

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  1. Yu Pang
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  2. Yage Zhang
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  3. Mengru Chen
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  4. Wei Lu
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  8. Wei Zhang
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Contributions

Z.Z. and W.Z. designed research and project outline. Y.P., Y.Z., MR.C., and M.C. performed isolation, deposition and polyphasic taxonomy. Y.P. and Y.Y. performed genome analysis. W.L. and W.Z. contributed to the polar lipid analysis. Y.P. and Z.Z. drafted the manuscript. M.L. revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wei Zhang or Zhengfu Zhou.

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Pang, Y., Zhang, Y., Chen, M. et al. Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea. Antonie van Leeuwenhoek 114, 1791–1804 (2021). https://doi.org/10.1007/s10482-021-01639-y

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