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Inactivation of Native K Channels

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The Journal of Membrane Biology Aims and scope Submit manuscript

…the fact that the potassium ion decreases rectification in the tissue may be of assistance in determining where in the cell rectification occurs… (Guttman 1944)

Abstract

We have experimented with isolated cardiomyocytes of mollusks Helix. During the whole-cell patch-clamp recordings of K+ currents a considerable decrease in amplitude was observed upon repeated voltage steps at 0.96 Hz. For these experiments, ventricular cells were depolarized to identical + 20 mV from a holding potential of − 50 mV. The observed spontaneous inhibition of outward currents persisted in the presence of 4-aminopyridine, tetraethylammonium chloride or E-4031, the selective class III antiarrhythmic agent that blocks HERG channels. Similar tendency was retained when components of currents sensitive to either 4-AP or TEA were mathematically subtracted. Waveforms of currents sensitive to 1 and 10 micromolar concentration of E-4031 were distinct comprising prevailingly those activated during up to 200 ms pulses. The outward current activated by a voltage ramp at 60 mV x s−1 rate revealed an inward rectification around + 20 mV. This feature closely resembles those of the mammalian cardiac delayed rectifier IKr.

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Abbreviations

4-AP:

4-Aminopyridine

HP:

Holding potential

I K :

Delayed rectifier K current and channel

Kv:

Voltage-dependent potassium channels

MP:

Membrane potential

TEA:

Tetraethylammonium chloride

TP:

Test pulse

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Acknowledgements

The support of Deutsche Forschungsgemeinschaft within SFB320 "Herzfunktion und ihre Regulation" is gratefully acknowledged. The authors greatly appreciate the excellent technical support of Isolde Villhauer and Klara Güth.

Funding

The funder was funded by Deutsche Forschungsgemeinschaft.

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Authors and Affiliations

  1. Department of Cardiology, Medical University Hospital Heidelberg, 69120, Heidelberg, Germany

    Sodikdjon A. Kodirov, Johannes Brachmann & Vladimir L. Zhuravlev

  2. Department of General Physiology, Saint Petersburg University, 199034, Saint Petersburg, Russia

    Sodikdjon A. Kodirov, Tatiana A. Safonova & Vladimir L. Zhuravlev

  3. Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, USA

    Sodikdjon A. Kodirov

  4. Department of Biophysics, Saint Petersburg University, 199034, Saint Petersburg, Russia

    Sodikdjon A. Kodirov

  5. Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Portugal

    Sodikdjon A. Kodirov

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  1. Sodikdjon A. Kodirov
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  2. Johannes Brachmann
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  3. Tatiana A. Safonova
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  4. Vladimir L. Zhuravlev
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Correspondence to Sodikdjon A. Kodirov.

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Kodirov, S.A., Brachmann, J., Safonova, T.A. et al. Inactivation of Native K Channels. J Membrane Biol 255, 13–31 (2022). https://doi.org/10.1007/s00232-021-00195-w

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