Abstract
Nanocomposite hydrogel is helpful to provide a moist and ideal environment for wound healing. In this research study, a nanocomposite hydrogel was prepared based on schizophyllan (SPG) and amine-functionalized bacterial cellulose (amine-functionalized BC). ZnO-nanoparticles were loaded into amine-grafted BC/SPG hydrogel to improve its antibacterial feature. The successful functionalization of bacterial cellulose by (3-aminopropyl)triethoxysilane was verified by FTIR and XRD analyses. XRD pattern of amine-grafted BC/SPG/ZnO NP showed some new peaks in comparison with XRD pattern of amine-grafted BC/SPG which verified the intercalation of ZnO into polymeric matrix. SEM images showed that the porous spaces of BC have been filled by schizophyllan as a result of the intermolecular attraction between them. Deposition of ZnO nanoparticles on the surface of amine-grafted BC/SPG has also been verified by SEM analysis. Amine-grafted BC/SPG membrane showed the higher swelling degree (850 ± 48%) in comparison with BC (512 ± 29%). The swelling ratio decreased to 725 ± 19% by addition of ZnO nanoparticles. Amine-grafted BC/SPG exhibited the higher mechanical strength (45 MPa) in comparison with pristine BC (10 MPa). The tensile strength of amine-grafted BC/SPG/ZnO NP nanocomposite reached ~ 70 MPa that might be resulted from the formation of hydrogen bonding between hydroxyl groups of polymers and oxygen atoms in the ZnO NPs. Amine-grafted BC/SPG/ZnO showed a more remarkable antibacterial efficiency against E. coli and S. aureus. As compared to BC, amine-grafted BC/SPG could stimulate the proliferation of human fibroblast cell. Cell viability showed a slight decrease after exposure to amine-grafted BC/SPG/ZnO NP nanocomposite.
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This research was funded by the Iran National Science Foundation (INSF) under grant number 96004235.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by SH and SAS. SH wrote the first draft of the manuscript and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Hamedi, S., Shojaosadati, S.A. Preparation of antibacterial ZnO NP-containing schizophyllan/bacterial cellulose nanocomposite for wound dressing. Cellulose 28, 9269–9282 (2021). https://doi.org/10.1007/s10570-021-04119-8
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DOI: https://doi.org/10.1007/s10570-021-04119-8