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Cold Atmosphere Plasma Modification on Beta-Carotene-Loaded Nanofibers to Enhance Osteogenic Differentiation

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An Erratum to this article was published on 01 October 2022

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Abstract

Since bone disorders have globally increased, tissue engineering could provide a solution by generating fully functional bone tissues. The most powerful aspect of bone tissue engineering is biomaterials. The focus of this study was the development of PCL nanofibers loaded with 4% β-carotene (βC) and PLGA nanofibers loaded with 2% βC as suitable bioactive scaffolds able to support the osteogenic differentiation of human bone marrow mesenchymal stem cells (MSCs). βC, a vitamin A progenitor, provided the potential for stimulating osteoblast differentiation. The electrospun PLGA and PCL nanofibers containing βC were treated by cold atmospheric plasma (CAP) at different times. These modified scaffolds were characterized by SEM to find the optimal time for CAP treatment. FTIR and contact angle measurements were used to detect and confirm surface chemical changes. Optimal CAP-treated scaffolds were seeded by MSCs and incubated for 21 days. The growth and proliferation of MSCs were analyzed by MTT assay in the early stages (up to 72 hours). The results confirmed the biocompatibility of the scaffolds. Our in-vitro study showed that the cell attachment, proliferation, and calcium deposition, as well as, the expression of RUNX2, SOX9, and osteonectin genes bone-specific markers during a 21-day culture were enhanced on CAP-treated PLGA/βC2% and PCL/βC4% nanofibers without an external bone differential agent. These advanced scaffolds can be applied in bone tissue engineering.

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Authors and Affiliations

  1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran

    Yasamin Moradi, Shiva Irani & Neda Dadgar

  2. Department of Mechanical Engineering, University of Tehran, Tehran, 1417466191, Iran

    Seyed Alireza Atyabi

  3. Department of Chemistry, Michigan State University, East Lansing, MI, 48824-1322, USA

    Ali Ghiassadin

  4. Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP, 14476, Potsdam, Germany

    Hadi Bakhshi

  5. Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, 1316943551, Iran

    Seyed Mohammad Atyabi

Authors
  1. Yasamin Moradi
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  2. Seyed Alireza Atyabi
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  3. Ali Ghiassadin
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  4. Hadi Bakhshi
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  5. Shiva Irani
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  6. Seyed Mohammad Atyabi
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  7. Neda Dadgar
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Correspondence to Hadi Bakhshi or Shiva Irani.

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Moradi, Y., Atyabi, S.A., Ghiassadin, A. et al. Cold Atmosphere Plasma Modification on Beta-Carotene-Loaded Nanofibers to Enhance Osteogenic Differentiation. Fibers Polym 23, 18–27 (2022). https://doi.org/10.1007/s12221-021-0033-y

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  • DOI: https://doi.org/10.1007/s12221-021-0033-y

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