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Identification and genome analysis of Comamonas testosteroni strain JLU460ET, a novel steroid-degrading bacterium

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Abstract

In this work, C. testosteroni JLU460ET isolated from animal waste was confirmed to have great degradation capability for 17β-estradiol and testosterone. This bacterium could degrade nearly 90% of 17β-estradiol (5 mg L−1) in 4 days and transform it into estrone for further degradation. One hundred percent testosterone (144 mg L−1) could be completely degraded after 9 h of incubation. This is the first report of C. testosteroni strains with the ability to degrade both estrogens and testosterone. The whole genome sequence of C. testosteroni JLU460ET was obtained and annotated, containing one chromosome (5,497,097 bp) with 61.37% GC content. A total of 4805 protein-coding genes and 134 RNA genes (including 29 rRNA genes, 102 tRNA genes and three ncRNA genes) were identified. Furthermore, the complete genome sequence of C. testosteroni JLU460ET was compared with four other C. testosteroni strains. Altogether, these five C. testosteroni strains contain 3508 core genes and 7616 pan genes. A steroid degradation pathway including 11 steroid degradation genes exists in core genes of five C. testosteroni strains. Twenty-two steroid degradation genes were found in the C. testosteroni JLU460ET genome, which has the most reported steroid degradation genes among the five C. testosteroni genomes. Further functional genomic analysis identified a gene cluster responsible for testosterone degradation in C. testosteroni JLU460ET, as well as a gene encoding 17β-HSD, the key enzyme for transforming 17β-estradiol into estrone. This work could enrich the genome sources of steroid-degrading strains and promote the study of steroid-degradation mechanism in bacteria.

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Funding

This study was supported by the National Science Foundation of China (Grant no. 32072924 and 31702299).

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Authors and Affiliations

  1. Key Laboratory of Groundwater Resources and Environment, Ministry of Education, College of New Energy and Environment, Jilin University, Changchun, 130021, China

    Na Liu, Yue-e Shi, Jialu Li, Meiling Zhu & Tingdi Zhang

Authors
  1. Na Liu
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  2. Yue-e Shi
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  3. Jialu Li
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  4. Meiling Zhu
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  5. Tingdi Zhang
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Contributions

TZ designed the experiments and wrote the manuscript. NL performed the experiments. YS, JL and MZ assisted the experiments. All the authors discussed the results and commented on the manuscript.

Corresponding author

Correspondence to Tingdi Zhang.

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The authors declare no competing interests.

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This article does not contain data from any study with human participants or animals.

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Liu, N., Shi, Ye., Li, J. et al. Identification and genome analysis of Comamonas testosteroni strain JLU460ET, a novel steroid-degrading bacterium. 3 Biotech 11, 404 (2021). https://doi.org/10.1007/s13205-021-02949-8

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  • DOI: https://doi.org/10.1007/s13205-021-02949-8

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