Skip to main content
SpringerLink
Log in

Suspension array for multiplex immunoassay of five common endocrine disrupter chemicals

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A low cost and effective indirect competitive method is reported to detect five EDCs, 17-beta-estradiol (E2), estriol (E3), bisphenol A (BPA), diethylstilbestrol (DES), and nonylphenol (NP) simultaneously, based on suspension array technology (SAT). Five kinds of complete antigens (E2-BSA, E3-BSA, BPA-BSA, DES-BPA, NP-BSA) were coupled to different encoding microspheres using purpose-made solutions in our laboratory instead of commercially available amino coupling kits; the method was further optimized for determination and reducing  the cost. Encoding and signaling fluorescence of the particles are determined at 635/532 nm emission wavelengths. High-throughput curves of five EDCs were draw and the limit of detection (LOD) were between 0.0010 ng mL−1 ~ 0.0070 ng mL−1. Compared with traditional ELISA methods, the SAT exhibited better specificity and sensitivity. Experiments using spiked milk and tap water samples were also carried out, and the recovery was between 85 and 110%; the results also confirmed good repeatability and reproducibility. It illustrated great potential of the present strategy in the detection of EDCs in actual samples.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Nelson W, Wang Y-X, Sakwari G, Ding Y-B (2020) Review of the effects of perinatal exposure to endocrine-disrupting chemicals in animals and humans. Rev Environ Contam Toxicol 251:131–184

    CAS  PubMed  Google Scholar 

  2. Gingrich J, Ticiani E, Veiga-Lopez A (2020) Placenta disrupted: endocrine disrupting chemicals and pregnancy. Trends Endocrinol Metab 31(7):508–524

    Article  CAS  Google Scholar 

  3. Hadibarata T, Kristanti RA, Mahmoud AH (2020) Occurrence of endocrine-disrupting chemicals (EDCs) in river water and sediment of the Mahakam River. J Water Health 18(1):38–47

    Article  Google Scholar 

  4. Liu Z-H, Dang Z, Yin H, Liu Y (2021) Making waves: improving removal performance of conventional wastewater treatment plants on endocrine disrupting compounds (EDCs): their conjugates matter. Water Res 188:116469

    Article  CAS  Google Scholar 

  5. Gore AC, Zoeller RT, Soto AM, Prins GS, Hauser R, Giudice LC, Bourguignon J-P, Diamanti-Kandarakis E (2009) Endocrine-disrupting chemicals: an Endocrine Society scientific statement. Endocr Rev 30(4):293–342

    Article  Google Scholar 

  6. Li J, Zhang W, Zhao H, Zhou Y, Xu S, Li Y, Xia W, Cai Z (2020) Trimester-specific, gender-specific, and low-dose effects associated with non-monotonic relationships of bisphenol A on estrone, 17β-estradiol and estriol. Environ Int 134:105304

    Article  CAS  Google Scholar 

  7. Korshin GV, Kim J, Gan L (2006) Comparative study of reactions of endocrine disruptors bisphenol A and diethylstilbestrol in electrochemical treatment and chlorination. Water Res 40(5):1070–1078

    Article  CAS  Google Scholar 

  8. Zhang L, Wei C, Zhang H, Song M (2017) Criteria for assessing the ecological risk of nonylphenol for aquatic life in Chinese surface fresh water. Chemosphere 184:569–574

    Article  CAS  Google Scholar 

  9. Rhind SM, Kyle CE, Mackie C, Telfer G (2007) Effects of exposure of ewes to sewage sludge-treated pasture on phthalate and alkyl phenol concentrations in their milk. Sci Total Environ 383(1–3):70–80

    Article  CAS  Google Scholar 

  10. Xu B, Li K, Qiao J, Liungai Z, Chen C, Lu Y (2017) UV photoconversion of environmental oestrogen diethylstilbestrol and its persistence in surface water under sunlight. Water Res 127:77–85

    Article  CAS  Google Scholar 

  11. Raymundo-Pereira PA, Campos AM, Vicentini FC, Janegitz BC, Mendonça CD, Furini LN, Boas NV, Calegaro ML, Constantino CJL, Machado SAS, Oliveira ON (2017) Sensitive detection of estriol hormone in creek water using a sensor platform based on carbon black and silver nanoparticles. Talanta 174:652–659

    Article  CAS  Google Scholar 

  12. He X, Mei X, Wang J, Lian Z, Tan L, Wu W (2016) Determination of diethylstilbestrol in seawater by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography. Mar Pollut Bull 102(1):142–147

    Article  CAS  Google Scholar 

  13. Yuan K, Kang H, Yue Z, Yang L, Lin L, Wang X, Luan T (2015) Determination of 13 endocrine disrupting chemicals in sediments by gas chromatography–mass spectrometry using subcritical water extraction coupled with dispersed liquid–liquid microextraction and derivatization. Anal Chim Acta 866:41–47

    Article  CAS  Google Scholar 

  14. Reale E, Fustinoni S, Mercadante R, Polledri E, Hopf NB (2020) Simultaneous quantification of bisphenol A, its Glucuronide metabolite, and commercial alternatives by LC-MS/MS for in vitro skin absorption evaluation. Chem Res Toxicol 33(9):2390–2400

    Article  CAS  Google Scholar 

  15. Jiang D, Cao B, Wang M, Yang H, Zhao K, Li J, Li M, Sun L, Deng A (2017) Development of a highly sensitive and specific monoclonal antibody based enzyme-linked immunosorbent assay for the detection of a new beta-agonist, phenylethanolamine A, in food samples. J Sci Food Agric 97(3):1001–1009

    Article  CAS  Google Scholar 

  16. Silva CP, Carvalho T, Schneider RJ, Esteves VI, Lima DLD (2020) ELISA as an effective tool to determine spatial and seasonal occurrence of emerging contaminants in the aquatic environment. Anal Methods 12(19):2517–2526

    Article  CAS  Google Scholar 

  17. Wu L, Li G, Xu X, Zhu L, Huang R, Chen X (2019) Application of nano-ELISA in food analysis: recent advances and challenges. TrAC Trends Anal Chem 113:140–156

    Article  CAS  Google Scholar 

  18. Xu Y, Yang H, Huang Z, Li Y, He Q, Tu Z, Ji Y, Ren W (2018) A peptide/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed. Food Chem 268:242–248

    Article  CAS  Google Scholar 

  19. Rhyne PW, Scull JD, Stiles LM, Eisinger DP (2003) Analysis of apoptotic cells using Beadlyte® suspension arrays. BioTechniques 35(3):624–629

    Article  CAS  Google Scholar 

  20. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR (1997) Advanced multiplexed analysis with the FlowMetrixTM system. Clin Chem 43:1749–1756

    Article  CAS  Google Scholar 

  21. Wang X, Gong J, Yuan B, Chen Z, Jiang J (2019) Sensitive and multiplexed detection of antibiotics using a suspension array platform based on silica-agarose hybrid microbeads. J Hazard Mater 373:115–121

    Article  CAS  Google Scholar 

  22. Kase JA, Maounounen-Laasri A, Lin A (2016) Rapid identification of Shiga toxin–producing Escherichia coli O serogroups from fresh produce and raw milk enrichment cultures by Luminex bead–based suspension array. J Food Prot 79(9):1623–1629

    Article  CAS  Google Scholar 

  23. Liu N, Gao Z, Ma H, Su P, Ma X, Li X, Ou G (2013) Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology. Biosens Bioelectron 41:710–716

    Article  CAS  Google Scholar 

  24. Wang X, Mu Z, Shangguan F, Liu R, Pu Y, Yin L (2014) Rapid and sensitive suspension array for multiplex detection of organophosphorus pesticides and carbamate pesticides based on silica–hydrogel hybrid microbeads. J Hazard Mater 273:287–292

    Article  CAS  Google Scholar 

  25. Fan R, Tang S, Luo S, Liu H, Zhang W, Yang C, He L, Chen Y (2020) Duplex surface enhanced Raman scattering-based lateral flow immunosensor for the low-level detection of antibiotic residues in milk. Molecules 25(22):5249

    Article  CAS  Google Scholar 

  26. Liu X, Fan X, Matsumoto H, Nie Y, Sha Z, Yi K, Pan J, Qian Y, Cao M, Wang Y, Zhu G, Wang M (2018) Biotoxin tropolone contamination associated with nationwide occurrence of pathogen Burkholderia plantarii in agricultural environments in China. Environ Sci Technol 52(9):5105–5114

    Article  CAS  Google Scholar 

  27. Zhu P, Fu W, Wei S, Liu X, Wang C, Lu Y, Shang Y, Wu X, Wu Y, Zhu S (2019) A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator. Sci Rep 9(1):7311

    Article  Google Scholar 

  28. Jia X-X, Yao Z-Y, Gao Z-X, Fan Z-C (2021) The role of suspension array technology in rapid detection of foodborne pollutants: applications and future challenges. Crit Rev Anal Chem:1–14

  29. Liu N, Wu J, Xianyu Y, Liang W, Li Y, Deng L, Chen Y (2020) Carbon nanotube–mediated antibody-free suspension array for determination of typical endocrine-disrupting chemicals. Microchim Acta 187(202)

  30. Graham H, Chandler DJ, Dunbar SA (2019) The genesis and evolution of bead-based multiplexing. Methods 158:2–11

    Article  CAS  Google Scholar 

  31. Jia Y, Peng Y, Bai J, Zhang X, Cui Y, Ning B, Cui J, Gao Z (2018) Magnetic nanoparticle enhanced surface plasmon resonance sensor for estradiol analysis. Sensors Actuators B Chem 254:629–635

    Article  CAS  Google Scholar 

  32. Wang J-Y, Guo Q-Y, Yao Z-Y, Yin N, Ren S-Y, Li Y, Li S, Peng Y, Bai J-L, Ning B-A, Liang J, Gao Z-X (2020) A low-field nuclear magnetic resonance DNA-hydrogel nanoprobe for bisphenol A determination in drinking water. Microchim Acta 187:333–340

    Article  CAS  Google Scholar 

  33. Mo F, Xie J, Wu T, Liu M, Zhang Y, Yao S (2019) A sensitive electrochemical sensor for bisphenol A on the basis of the AuPd incorporated carboxylic multi-walled carbon nanotubes. Food Chem 292:253–259

    Article  CAS  Google Scholar 

  34. Hou C, Zhao L, Geng F, Wang D, Guo L-H (2016) Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol a. Anal Bioanal Chem 408:8795–8804

    Article  CAS  Google Scholar 

  35. Hosseini S, Vázquez-Villegas P, Rito-Palomares M, Martinez-Chapa SO (2018) Advantages, Disadvantages and Modifications of Conventional ELISA, pp 67–115

    Google Scholar 

  36. Stewart A, Banerji U (2017) Utilizing the Luminex magnetic bead-based suspension array for rapid multiplexed phosphoprotein quantification. Methods Mol Biol 1636:119–131

    Article  CAS  Google Scholar 

  37. Wee SY, Aris AZ, Yusoff FM, Praveena SM (2019) Occurrence and risk assessment of multiclass endocrine disrupting compounds in an urban tropical river and a proposed risk management and monitoring framework. Sci Total Environ 671:431–442

    Article  CAS  Google Scholar 

  38. Wee SY, Aris AZ, Yusoff FM, Praveena SM (2021) Tap water contamination: multiclass endocrine disrupting compounds in different housing types in an urban settlement. Chemosphere 264:128488

    Article  CAS  Google Scholar 

  39. Kasonga TK, Coetzee MAA, Kamika I, Ngole-Jeme VM, Benteke Momba MN (2021) Endocrine-disruptive chemicals as contaminants of emerging concern in wastewater and surface water: a review. J Environ Manag 277:111485

    Article  CAS  Google Scholar 

  40. Solecki R, Kortenkamp A, Bergman Ak, Chahoud I, Degen GH, Dietrich D, Greim H, Håkansson H, Hass U, Husoy T, Jacobs M, Jobling S, Mantovani A, Marx-Stoelting P, Piersma A, Ritz V, Slama R, Stahlmann R, van den Berg M, Zoeller RT, Boobis AR (2017) Scientific principles for the identification of endocrine-disrupting chemicals: a consensus statement. Arch Toxicol 91(2):1001–1006

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the [National key R & D projects of China] under Grant [No.2017YFF0211202].

Author information

Authors and Affiliations

  1. College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic of China

    Xue-xia Jia

  2. Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, 1 Da Li Road, Tianjin, 300050, People’s Republic of China

    Xue-xia Jia, Zi-yi Yao, Sha Liu & Zhi-xian Gao

Authors
  1. Xue-xia Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zi-yi Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sha Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhi-xian Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhi-xian Gao.

Ethics declarations

Conflict of interest

None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

ESM 1

(DOCX 95.6 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jia, Xx., Yao, Zy., Liu, S. et al. Suspension array for multiplex immunoassay of five common endocrine disrupter chemicals. Microchim Acta 188, 290 (2021). https://doi.org/10.1007/s00604-021-04905-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-021-04905-y

Keywords

Search

Navigation