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The Microbial Community of Poultry Farm Waste and Its Role in Hydrogen Sulfide Production

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Abstract

The microbiota of chicken litter remains largely unexplored, despite its leading role in the formation of volatile odorants and unpleasant odors. One of the main components of the odor is hydrogen sulfide, which may be formed as a result of microbial sulfate reduction. The composition of the microbial community of poultry plant waste was investigated. Samples were collected at the Mezheninovskaya large poultry farm, 15 km from the city of Tomsk, Russia. The 16S rRNA gene profiling revealed sulfate-reducing Desulfovibrionaceae in the microbial community. Both their share in the community and sulfide production increased upon incubation of the waste in the medium with sulfate and lactate. The possible sulfate sources in the waste are gypsum and the feed additives introduced as sulfates, such as lysine sulfate.

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Funding

This work was in part supported by the Russian Foundation for Basic Research, project no. 19-14-00245, and the Ministry of Science and Higher Education of Russia.

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Authors and Affiliations

  1. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    E. V. Gruzdev, A. V. Beletsky, A. V. Mardanov & N. V. Ravin

  2. Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050, Tomsk, Russia

    E. A. Latygolets, M. A. Grigoriev, M. K. Kadyrbaev, O. P. Ikkert & O. V. Karnachuk

Authors
  1. E. V. Gruzdev
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  2. E. A. Latygolets
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  3. A. V. Beletsky
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  4. M. A. Grigoriev
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  5. A. V. Mardanov
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  6. M. K. Kadyrbaev
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  7. O. P. Ikkert
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  8. O. V. Karnachuk
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  9. N. V. Ravin
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Corresponding author

Correspondence to E. V. Gruzdev.

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COMPLIANCE WITH ETHICAL STANDARDS

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHOR CONTRIBUTION

Sampling and microcosm experiments were carried out by E.A. Latygolets and M.A. Grigoriev. M. Kadyrbaev measured the hydrogen sulfide concentration. O.P. Ikkert performed diffraction and elemental analysis. Isolation of DNA from microcosms and the 16S rRNA gene sequencing were carried out by E.V. Gruzdev and A.V. Mardanov. Bioinformatic analysis of sequences was carried out by A.V. Beletsky. Data analysis and preparation of the article were performed by O.V. Karnachuk and N.V. Ravin. All authors participated in the discussion of the results.

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Translated by E. Babchenko

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Gruzdev, E.V., Latygolets, E.A., Beletsky, A.V. et al. The Microbial Community of Poultry Farm Waste and Its Role in Hydrogen Sulfide Production. Microbiology 90, 507–511 (2021). https://doi.org/10.1134/S0026261721040044

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  • DOI: https://doi.org/10.1134/S0026261721040044

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