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Species Delimitation and Cryptic Diversity Analysis of Parachlorella-Сlade Microalgae (Chlorophyta)

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Abstract—

This article discusses the system of green microalgae of the clade Parachlorella, based on morphological, ecological, and molecular genetic data. The diagnostic characteristics of the selected genera and species and the systematic position of some taxa are discussed. Phylogenetic analysis of the Parachlorella-clade has shown that, despite the relative simplicity of morphology, it is characterized by high cryptic diversity. The polyphyletic nature of the genus Dictyosphaerium and some species (D. libertatis, D. ehrenbergianum, Compactochlorella kochii, Mucidosphaerium pulchellum, M. palustre, and Closteriopsis acicularis) was shown. Theuse of the intron as a universal criterion for closely related species was effective for representatives of groups II, IV, VII, and VIII, as well as for strains of the species Compactochlorella kochii and D. libertatis. The application of the classical CBC approach, based on the search for CBC in conservative ITS2 regions, was successful only for the representatives of group II. The results of analysis of genetic distances and the GMYC, PTP, and ABGD species delimitation algorithms are not universal tools for determining species boundaries and need to be confirmed by other characteristics (cell shape, ability to form colonies, the way the cells attach to hyaline interconnecting strands, the type of chloroplast, the number of pyrenoids, the presence of mucilage, the presence/absence of introns, the level of genetic differences, the presence of CBC in ITS1 and ITS2, the habitat). Due to the combined use of morphological, ecological and genetic features, it was possible to characterize 11 groups and 2 phylogenetic lines within the clade Parachlorella and to make assumptions about the division of genera and species within the selected groups.

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ACKNOWLEDGMENTS

The authors are grateful to M.A. Sinetova, the Chief of the IPPAS Collection of Microalgae and Cyanobacteria, the Institute of Plant Physiology, Russian Academy of Sciences, for providing the strains.

Funding

The cultivation and microscopy of ACSSI strains and literature analysis were supported by the Russian Foundation for Basic Research, project no. 19-34-60002; molecular phylogenetic studies were supported by the Russian Science Foundation, project no. 19-74-00030.

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Authors and Affiliations

  1. Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences – separate subdivision Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    E. S. Krivina & A. D. Temraleeva

  2. Limnological Institute, Siberian Branch, Russian Academy of Sciences, 664003, Irkutsk, Russia

    Yu. S. Bukin

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  1. E. S. Krivina
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Correspondence to E. S. Krivina.

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The authors declare that they have no conflict of interest. The article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Makeeva

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Krivina, E.S., Temraleeva, A.D. & Bukin, Y.S. Species Delimitation and Cryptic Diversity Analysis of Parachlorella-Сlade Microalgae (Chlorophyta). Microbiology 90, 455–469 (2021). https://doi.org/10.1134/S0026261721040081

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