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Benefits of Endophytic Fungus Phomopsis liquidambaris Inoculation for Improving Mineral Nutrition, Quality, and Yield of Rice Grains Under Low Nitrogen and Phosphorus Condition

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Abstract

The deficiency of N-P availability in soil is a primary cause for crop yield and nutrient loss. Since plant-beneficial microbial symbiosis is an eco-friendly method for compensating this negative effect, understanding the interconnection between endophytic fungus colonization and mineral nutrient accumulation in edible parts of crop is important in agricultural production. In this study, we focused on the effects of endophytic fungus Phomopsis liquidambaris on the absorption and distribution of 14 essential mineral elements in vegetative organs (root and shoot) and reproductive organs (grain) of rice during its growing period with reduced N-P fertilizer application, as well as the changes in yield, quality, and mineral nutrition of rice grains. The possible mechanisms including the transcriptional level of the transport-related genes and the structure of endophytic community were also investigated. The results indicated that the P. liquidambaris significantly increased the accumulation of N, P, Fe, Mn, Zn, Mo, and Se in rice grains by 20.5%, 18.9%, 18.2%, 9.5%, 17.9%, 13.3%, and 17.1%, respectively, accompanied by the significant increase in yield (23.8%) and protein content (17.3%). These beneficial effects were attributed to the enhancement formation of iron plaque, the upregulation of nutrient transporters genes, and the altered endophyte community by P. liquidambaris symbiosis. In conclusion, P. liquidambaris could promote the partition of nutrients to the grains by increasing the efficiency of nutrient uptake and remobilization. Our results suggest P. liquidambaris symbiosis as a useful candidate for improving the mineral nutrition, quality, and yield of rice grains under N-P-deficient field condition.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC No. 32071638) and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions. The authors express their great thanks to the editorial staff and reviewers for their time and attention.

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China

    Meng-Jun Tang, Fan Lu, Yang Yang, Kai Sun, Qiang Zhu, Fang-Ji Xu, Wei Zhang & Chuan-Chao Dai

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  1. Meng-Jun Tang
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  2. Fan Lu
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  3. Yang Yang
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  4. Kai Sun
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  6. Fang-Ji Xu
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  7. Wei Zhang
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  8. Chuan-Chao Dai
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Contributions

M-JT conducted all the experiments. M-JT and FL designed the experiments, discussed and analyzed the data, and wrote the paper. C-CD supervised the study and advised on experimental layout. YY, KS, QZ, F-JX, WZ, and YZ participated in preparing initial paper draft. The final version was read and approved by all authors.

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Correspondence to Chuan-Chao Dai.

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Tang, MJ., Lu, F., Yang, Y. et al. Benefits of Endophytic Fungus Phomopsis liquidambaris Inoculation for Improving Mineral Nutrition, Quality, and Yield of Rice Grains Under Low Nitrogen and Phosphorus Condition. J Plant Growth Regul 41, 2499–2513 (2022). https://doi.org/10.1007/s00344-021-10462-8

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