Skip to main content
SpringerLink
Log in

Selective Removal of Florfenicol from Fetal Bovine Serum by Restricted Access Media–Magnetic Molecularly Imprinted Polymers

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Unique restricted access media-magnetic molecularly imprinted polymers (RAM–MMIPs) were prepared as magnetic solid-phase extraction (MSPE) material for florfenicol (FF) and are combined with HPLC–UV to extract FF from fetal bovine serum samples. Under the optimization conditions, the resulting materials had a uniform imprinted layer, acceptable adsorption amounts (22.92 mg/g), rapid binding kinetics (80 min) and satisfactory selectivity. In addition, RAM–MMIPs presented satisfactory recoveries (95.6–102.1%), relative standard deviation (RSD) (2.7–3.9%) and lower limits of detection (LOD) (10.5 μg/L). The method was applied to the real samples. Compared with other reported methods, this method exhibits the advantages of simple, fast and, efficient operation, using a relatively small amount of the RAM–MMIPs adsorbent. The developed method can enrich FF from fetal bovine serum samples.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data Availability

The data used to support the finding of this study are available from the corresponding author upon request.

Code Availability

Not applicable.

References

  1. Sadeghi S, Olieaei S (2019) Capped cadmium sulfide quantum dots with a new ionic liquid as a fluorescent probe for sensitive detection of florfenicol in meat samples. Spectrochim Acta A 223:117349

    Article  CAS  Google Scholar 

  2. Yuan XH, Liu J, Li RL, Zhou JL, Wei JH, Jiao SM, Wang ZA (2020) Chitosan oligosaccharides coupling inhibits bacterial biofilm-related antibiotic resistance against florfenicol. Molecules 25(24):6043

    Article  CAS  Google Scholar 

  3. Gaikowski MP, Mushtaq M, Cassidy P, Meinertz JR, Schlies SM, Sweeney D, Endris RG (2010) Depletion of florfenicol amine, marker residue of florfenicol, from the edible fillet of tilapia (oreochromis niloticus x o. niloticus and o. niloticus x o. aureus) following florfenicol administration in feed. Aquaculture 301(1–4):1–6

    Article  CAS  Google Scholar 

  4. Barreto F, Ribeiro C, Hoff RB, Costa TD (2016) Determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in poultry, swine, bovine and fish by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1449:48–53

    Article  CAS  Google Scholar 

  5. Blondeau JM, Shebelski SD, Hesje CK (2015) Bactericidal effects of various concentrations of enrofloxacin, florfenicol, tilmicosin phosphate, and tulathromycin on clinical isolates of mannheimia haemolytica. Am J Vet Res 76(10):860–868

    Article  CAS  Google Scholar 

  6. China PR (2002) Veterinary drug maximum residue limits in the food of animal origin Z. Bull Minist Agric 12:235

    Google Scholar 

  7. Shida SS, Shiono K, Narushima J, Nemoto S, Akiyama H (2019) Determination of total florfenicol residues as florfenicol amine in bovine tissues and eel by liquid chromatography-tandem mass spectrometry using external calibration. J Chromatogr B 1109:37–44

    Article  Google Scholar 

  8. Dudoit A, Mertz C, Chillet M, Chillet M, Cardinault N, Brat P (2020) Antifungal activity of brazilian red propolis extract and isolation of bioactive fractions by thin-layer chromatography-bioautography. Food Chem 327:127060

    Article  CAS  Google Scholar 

  9. Lodi G, Pellegrini LA, Aliverti A, Torres BR, Bernardi M, Morbidelli M, Storti G (2017) Recovery of monosaccharides from lignocellulosic hydrolysates by ion exclusion chromatography. J Chromatogr A 1496:25–36

    Article  CAS  Google Scholar 

  10. Yu KL, Yue ME, Xu J, Jiang TF (2020) Determination of fluoroquinolones in milk, honey and water samples by salting out-assisted dispersive liquid-liquid microextraction based on deep eutectic solvent combined with MECC. Food Chem 332:127371

    Article  CAS  Google Scholar 

  11. Sanchez OA, Sampedro MC, Unceta N, Barrio RJ (2005) Solid-phase microextraction coupled with high performance liquid chromatography using on-line diode-array and electrochemical detection for the determination of fenitrothion and its main metabolites in environmental water samples. J Chromatogr A 1094(1–2):70–76

    Article  Google Scholar 

  12. Sadeghi S, Jahani M (2014) Solid-phase extraction of florfenicol from meat samples by a newly synthesized surface molecularly imprinted sol–gel polymer. Food Anal Method 7(10):2084–2094

    Article  Google Scholar 

  13. Cheng GH, Zhao J, Wang XY, Yang C, Li SY, Lu T, Li X, Wang XF, Zhu GF (2021) A highly sensitive and selective method for the determination of ceftiofur sodium in milk and animal-origin food based on molecularly imprinted solid-phase extraction coupled with HPLC-UV. Food Chem 347:129013

    Article  CAS  Google Scholar 

  14. Shakourian M, Yamini Y, Safari M, Safari, (2020) Facile magnetization of metal–organic framework TMU-6 for magnetic solid-phase extraction of organophosphorus pesticides in water and rice samples. Talanta 218:121139

    Article  CAS  Google Scholar 

  15. Pichon V, Delaunay N, Combes A (2019) Sample preparation using molecularly imprinted polymers. Anal Chem 92(1):16–33

    Article  Google Scholar 

  16. Fang L, Miao YX, Wei D, Zhang Y, Zhou YC (2021) Efficient removal of norfloxacin in water using magnetic molecularly imprinted polymer. Chemosphere 262:128032

    Article  CAS  Google Scholar 

  17. Riskin M, Amram BY, Vered RT, Chegel V, Almong J, Willner I (2011) Molecularly imprinted Au nanoparticles composites on Au surfaces for the surface plasmon resonance detection of pentaerythritol tetranitrate, nitroglycerin, and ethylene glycol dinitrate. Anal Chem 83:3082–3088

    Article  CAS  Google Scholar 

  18. Nováková L, Vlčková H (2009) A review of current trends and advances in modern bio-analytical methods: chromatography and sample preparation. Anal Chim Acta 656(1–2):8–35

    Article  Google Scholar 

  19. He J, Song L, Chen S, Li YY, Wei HL, Zhao DX, Zhang GuKR, SS, (2015) Novel restricted access materials combined to molecularly imprinted polymers for selective solid-phase extraction of organophosphorus pesticides from honey. Food Chem 187:331–337

    Article  CAS  Google Scholar 

  20. Wu WJ, Su SF, Jiang P, Wang HS, Dong XC, Zhang M (2010) Determination of sulfonamides in bovine milk with column-switching high performance liquid chromatography using surface imprinted silica with hydrophilic external layer as restricted access and selective extraction materical. J Chromatogr A 1217(46):7198–7207

    Article  Google Scholar 

  21. Liu H, Ding J, Zhang K, Ding L (2019) Fabrication of carbon dots@restricted access molecularly imprinted polymers for selective detection of metronidazole in serum. Talanta 209:120508

    Article  Google Scholar 

  22. Chen H, Son S, Zhang F, Yan J, Li Y, Ding H, Ding L (2015) Rapid preparation of molecularly imprinted polymers by microwave-assisted emulsion polymerization for the extraction of florfenicol in milk. J Chromatogr B 983–984:32–38

    Article  Google Scholar 

  23. Wang X, Zhao L, Sun Z (2019) Preparation of ofloxacin-restricted access media–molecularly imprinted polymers for its selective recognition of ofloxacin in milk samples. Chromatographia 82:1041–1050

    Article  CAS  Google Scholar 

  24. Ge SG, Yan M, Cheng XL, Zhang CC, Yu JH, Zhao PN, Gao WG (2010) On-line molecular imprinted solid-phase extraction flow-injection fluorescence sensor for determination of florfenicol in animal tissues. J Pharmaceut Biomed 52(4):615–619

    Article  CAS  Google Scholar 

  25. Jalilian N, Ebrahimzadeh H, Asgharinezhad AA, Khodayari P (2021) Magnetic molecularly imprinted polymer for the selective dispersive micro solid phase extraction of phenolphthalein in urine samples and herbal slimming capsules prior to HPLC-PDA analysis. Microchem J 160:105712

    Article  CAS  Google Scholar 

  26. Zhou YQ, Yang YH, Ma MH, Sun ZA, Wu SS, Gong BL (2018) Preparation of imazethapyr surface molecularly imprinted polymers for its selective recognition of imazethapyr in soil samples. J Anal Methods Chem 2018:1–9

    Article  Google Scholar 

  27. Chen F, Xiao Y, Zhang B, Chang R, Liu D (2019) Magnetically stabilized bed packed with synthesized magnetic silicone loaded with ionic liquid particles for efficient enrichment of flavonoids from tree peony petals. J Chromatogr A 1613:460671

    Article  Google Scholar 

  28. Liang T, Chen LG, Ma Y (2020) Mesoporous structured molecularly imprinted polymer with restricted access function for highly selective extraction of chlorpyrifos from soil. J Chromatogr A 1609:460453

    Article  CAS  Google Scholar 

  29. Lv K, He Y, Xiong X, Wang Z, Wang H, Han Y (2015) Layer-by-layer fabrication of restricted access media-molecularly imprinted magnetic microspheres for magnetic dispersion microextraction of bisphenol A from milk samples. New J Chem 39(3):1792–1799

    Article  CAS  Google Scholar 

  30. Du B, Qiu T, Chen Z, Cao X, Han S, Shen G, Wang L (2014) A novel restricted access material combined to molecularly imprinted polymers for selective solid-phase extraction and high performance liquid chromatography determination of 2-methoxyestradiol in plasma samples. Talanta 129:465–472

    Article  CAS  Google Scholar 

  31. Zhao SW, Sun ZA, Wang XX, Li JM, Zhou YQ, Gong BL (2020) Novel metal-organic framework combining with restricted access molecularly imprinted nanomaterials for solid-phase extraction of gatifloxacin from bovine serum. J Chromatogr B 1157:122338

    Article  CAS  Google Scholar 

  32. Vinh NT, Tuan LA, Vinh LK, Quy NV (2020) Synthesis, characterization, and gas sensing properties of Fe3O4/FeOOH nanocomposites for a mass-type gas sensor. Mat Sci Semicon Proc 118:460671

    Article  Google Scholar 

  33. Bin S, Wang A, Guo W, Yu L, Feng P (2020) Micro magnetic field produced by Fe3O4 nanoparticles in bone scaffold for enhancing cellular activity. Polymers-Basel 12(9):2045

    Article  CAS  Google Scholar 

  34. Cui Y, Kang WW, Qin L, Ma JH, Liu XG, Yang YZ (2020) Magnetic surface molecularly imprinted polymer for selective adsorption of quinoline from coking wastewater. Chem Eng J 397:125480

    Article  CAS  Google Scholar 

Download references

Funding

This work was financially supported by the Key Research and Development Program of Ningxia (Special Project for Foreign Cooperation) (No. 2019BFH02004) and the National Natural Science Foundation of China (No. 21565001).

Author information

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, No. 204 Wenchang North Street, Xixia District, Yinchuan, 750021, P. R. China

    Siyu Guo, Shanshan Wu, Shanwen Zhao, Xiaoxiao Wang, Tianpei Cai, Jianmin li & Bolin Gong

Authors
  1. Siyu Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shanshan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shanwen Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoxiao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tianpei Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jianmin li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Bolin Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors are contributed equally to this work.

Corresponding author

Correspondence to Bolin Gong.

Ethics declarations

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. The fetal bovine serum samples used in this paper was purchased from a commercial institute in China.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 296 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Guo, S., Wu, S., Zhao, S. et al. Selective Removal of Florfenicol from Fetal Bovine Serum by Restricted Access Media–Magnetic Molecularly Imprinted Polymers. Chromatographia 84, 845–854 (2021). https://doi.org/10.1007/s10337-021-04074-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-021-04074-3

Keywords

Search

Navigation