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Transcriptional regulation of small heat shock protein genes by heat shock factor 1 (HSF1) in Liriomyza trifolii under heat stress

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Cell Stress and Chaperones Aims and scope

Abstract

Small heat shock proteins (sHSPs) function as molecular chaperones in multiple physiological processes and are active during thermal stress. sHSP expression is controlled by heat shock transcription factor (HSF); however, few studies have been conducted on HSF in agricultural pests. Liriomyza trifolii is an introduced insect pest of horticultural and vegetable crops in China. In this study, the master regulator, HSF1, was cloned and characterized from L. trifolii, and the expression levels of HSF1 and five sHSPs were studied during heat stress. HSF1 expression in L. trifolii generally decreased with rising temperatures, whereas expression of the five sHSPs showed an increasing trend that correlated with elevated temperatures. All five sHSPs and HSF1 showed an upward trend in expression with exposure to 40 ℃ without a recovery period. When a recovery period was incorporated after thermal stress, the expression patterns of HSF1 and sHSPs in L. trifolii exposed to 40 °C was significantly lower than expression with no recovery period. To elucidate potential interactions between HSF1 and sHSPs, double-stranded RNA was synthesized to knock down HSF1 in L. trifolii by RNA interference. The knockdown of HSF1 by RNAi decreased the survival rate and expression of HSP19.5, HSP20.8, and HSP21.3 during high-temperature stress. This study expands our understanding of HSF1-regulated gene expression in L. trifolii exposed to heat stress.

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Acknowledgements

This research was funded by the earmarked fund for Jiangsu Agricultural Industry Technology System (JATS [2020] 309), the Jiangsu Science & Technology Support Program (BE2014410), and the postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_2374).

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Authors and Affiliations

  1. College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China

    Ya-Wen Chang, Yu-Cheng Wang, Xiao-Xiang Zhang, Junaid Iqbal, Ming-Xing Lu & Yu-Zhou Du

  2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou, China

    Yu-Zhou Du

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  1. Ya-Wen Chang
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Chang, YW., Wang, YC., Zhang, XX. et al. Transcriptional regulation of small heat shock protein genes by heat shock factor 1 (HSF1) in Liriomyza trifolii under heat stress. Cell Stress and Chaperones 26, 835–843 (2021). https://doi.org/10.1007/s12192-021-01224-2

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