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Isolation, identification, and resistance gene detection of Vibrio harveyi from Scophthalmus maximus

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Abstract

Pathogenic bacteria of Scophthalmus maximus enteritis were isolated from and identified in an aquaculture farm in Changli County, Hebei Province, China. The feasibility of rapid selection of prevention and treatment drugs via PCR technology was investigated through drug resistance phenotype analysis and drug resistance gene detection. A dominant bacterial strain was isolated from S. maximus and designated HC-1. Morphological observations, culture property investigations, and physiological-biochemical experiments were conducted, which showed that the strain was consistent with the phenotypic characteristics of Vibrio. Based on 16S rRNA sequence determination and homology analysis, HC-1 was determined to be 99.65% similar to Vibrio harveyi NR 043165.1. Therefore, the bacterium was identified as V. harveyi. The sensitivity of the V. harveyi isolate to 41 antibiotics was determined by the Kirby-Bauer method. The results showed that HC-1 was resistant to 8 agents, namely, penicillin, oxacillin, amoxicillin, ampicillin, spectinomycin, azithromycin, florfenicol, and carbenicillin. The antimicrobial resistance gene analysis results showed that tem, ant(3˝)-I and tetA are carried by the chromosome of the strain and the plasmid, while sul1 is carried by the chromosome of the strain but not the plasmid. In contrast, floR is carried only by the plasmid but not by the chromosome. vanA, qnrA, and mefA are not carried by the plasmid or chromosome of the strain. Although the resistant phenotype of the strains is regulated by resistance genes, the relationship between the phenotype and genotype should be revealed by numerous databases. To accelerate the speed of treatment selection, it is necessary to apply PCR technology to detect resistance genes to replace drug sensitivity testing. These results provide useful information for further understanding the antimicrobial resistance factors and treatment of bacterial disease in S. maximus.

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Acknowledgments

The authors of this manuscript are thankful to the Analysis and Testing Center of Hebei Normal University of Science and Technology for its kind support and help in preparing this review article.

Funding

This work was supported by the National Natural Science Foundation of China (31502187); the Major Science and Technology Projects of Hebei Province (202867012); the Natural Science Foundation of Hebei Province, China (C2018407049), the Science and Technology Projects of Hebei Province (20567621H); and the Marine Special Foundation of Hebei Normal University of Science and Technology (2018HY022), which are gratefully acknowledged.

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  1. Yongzhu Yuan and Yonggang Zhang contributed equally to this paper and should be considered as co-first author.

Authors and Affiliations

  1. Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science and Technology, Changli, 066600, Hebei, China

    Yongzhu Yuan, Guoshan Qi, Hai Ren, Guisheng Gao, Xiaomin Jin & Hai Fang

  2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, Shandong, China

    Yonggang Zhang

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  1. Yongzhu Yuan
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Contributions

Yongzhu Yuan wrote the rough draft of the manuscript.

Yonggang Zhang made detailed supplements and modifications to the whole article.

Mrs. Xiaomin Jin designed the experimental scheme, collected the experimental data, and coordinated the revision of the entire article.

Mr. Guoshan QI, Mr. Hai Fang, and Mr. Hai Ren made important contributions to the conception, design and modification of the article.

Corresponding authors

Correspondence to Xiaomin Jin or Hai Fang.

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Yuan, Y., Zhang, Y., Qi, G. et al. Isolation, identification, and resistance gene detection of Vibrio harveyi from Scophthalmus maximus. Aquacult Int 29, 2357–2368 (2021). https://doi.org/10.1007/s10499-021-00752-z

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