FormalPara Key Summary Points

Why carry out this study?

It is difficult to achieve target blood pressure (BP) goals in patients with chronic kidney disease (CKD) and uncontrolled hypertension.

Nifedipine gastrointestinal therapeutic system (GITS) 60 mg has shown effectiveness in patients with hypertension. However, studies in Chinese patients with CKD with nifedipine GITS 60 mg are limited.

We conducted this study to evaluate the effectiveness and tolerability of nifedipine GITS 60 mg in Chinese patients with CKD and uncontrolled hypertension in real-world clinical settings.

What was learned from the study?

In Chinese patients with CKD and uncontrolled hypertension, nifedipine GITS 60 mg showed effectiveness in reducing office systolic BP (SBP) and diastolic BP (DPB); the reduction of SBP/DBP was positively correlated with baseline BP and was not affected by different stages of CKD. The safety analysis revealed its tolerability.

Nifedipine GITS 60 mg might play an essential role in improving the hypertension management practice in patients with CKD, and offer a new therapeutic option.

Introduction

The prevalence of chronic kidney disease (CKD) is gradually increasing, and as per 2017 data, there were 1.2 million deaths that were attributed to CKD globally [1]. Untreated or inadequately controlled hypertension is considered as one of the most important risk factors for the progression of CKD [2], leading to the development of end-stage renal disease (ESRD) in both men and women [3]. Among patients with CKD, hypertension is the most common comorbidity, reported in 67–92% of patients with CKD [4]. In China, a nationwide survey conducted in the period 2009 to 2010 showed the prevalence of CKD to be 10.8% (119.5 million), whereas only 12.5% of them were aware of their condition [5, 6]. As per the multicenter study conducted in China, 33.1% of the patients achieved the target blood pressure (BP) control rate of < 140/90 mmHg and 14.1% of the patients achieved the target of < 130/80 mmHg, suggesting a suboptimal control of hypertension in patients with CKD [6].

Treatment of hypertension in patients with CKD to achieve a BP target goal is challenging as they often have severe hypertension, which requires the use of multiple medications or large dosage of antihypertensive agents to achieve target BP goals [7]. The Eighth Joint National Committee (JNC8) guidelines recommend the use of thiazide-type diuretics, calcium channel blockers (CCBs), angiotensin-converting enzyme inhibitors (ACEI), or angiotensin receptor blockers (ARB) as the initial treatment agents for hypertension [8]. CCBs are the most commonly used antihypertensive drugs for Chinese patients with CKD (78%), the second and third being ARB (42.2%) and ACEI (18.0%), respectively, and 16.6% of patients use diuretics [9]. Several placebo-controlled trials in China employed a dihydropyridine CCB as the first-line drug for the treatment of active hypertension [10, 11].

Nifedipine is the prototype of dihydropyridine CCBs and is widely used as first-line therapy in patients with hypertension [12]. However, short-acting, immediate-release formulation required multiple daily dosing and caused rapid vasodilation followed by reflex sympathetic activation, resulting in side effects such as headaches, palpitations, and flushing [13]. This led to the launch of the extended-release preparations. The nifedipine gastrointestinal therapeutic system (GITS) is a double-coated bilayer tablet, which provides a constant release rate for approximately 20–22 h via a membrane-controlled, osmotic push–pull process [14]. Furthermore, the GITS dosage form provides a relatively constant plasma nifedipine concentration–time profile throughout the 24-h dosing interval with very little peak to trough fluctuation and renal impairment does not affect the half-life [15]. Additionally, no substantial changes with regard to plasma concentrations or bioavailability of nifedipine were detected in patients with impaired kidney function compared with healthy volunteers [16]; hence, dosage adjustment of nifedipine GITS is not required in patients with chronic renal impairment.

The Chinese general practice clinical guideline/expert consensus on the application of long-acting dihydropyridine CCBs in patients with CKD and hypertension recommended that long-acting CCBs (e.g., nifedipine GITS) can protect the renal function and play an important role in the treatment of renal failure [17]. The previously conducted studies such as FOCUS [18] and EXACT trial [19] have demonstrated the efficacy of nifedipine GITS 60 mg very well. However, clinical studies with nifedipine 60 mg in Chinese patients with CKD are limited. We aimed to provide the relevant clinical experience to physicians with nifedipine 60 mg. Hence, we conducted this prospective observational study to assess the effectiveness and tolerability of nifedipine GITS 60 mg in a large cohort of patients with CKD and uncontrolled hypertension in real-world clinical settings in China.

Methods

Study Design and Population

A prospective, multicenter, phase 4, observational study was conducted in 871 patients with CKD and uncontrolled hypertension (office systolic blood pressure [SBP] of ≥ 140 mmHg and diastolic blood pressure [DBP] of ≥ 80 mmHg who have received renin–angiotensin system inhibitors (RASIs) or have not received RASIs treatment because of any contraindications), aged 18–70 years from 17 nephrology clinics across China from July 2017 to August 2020 (ClinicalTrials.gov identifier NCT03194633). The study was approved by the ethics committee of First Affiliated Hospital, College of Medicine, Zhejiang University, 2016 Lun Shen No. (80), which is the master ethics committee. Approval was also provided by the institutional review board of the participating study centers (Table S1 in the supplementary material).The study was conducted in accordance with the Helsinki Declaration of 1964 and its later amendments. All patients provided written informed consent before the study initiation.

The inclusion criteria for the study were (1) male and female patients aged 18–70 years; (2) patients diagnosed with CKD (estimated glomerular filtration rate [eGFR] > 15 mL/min/1.73 m2) and hypertension without dialysis/renal replacement therapy; (3) patients with uncontrolled hypertension (office [SBP] ≥ 140 mmHg and [DBP] ≥ 80 mmHg) who have received (RASIs) or have not received RASI treatment because of any contraindications; (4) patients who have not received nifedipine GITS 60 mg (once per day) previously; (5) patients for whom the decision to initiate nifedipine GITS 60 mg treatment was made as per the investigator’s routine treatment practice; (6) those who provided signed informed consent; and (7) who have not participated in an investigational program with interventions outside of routine clinical practice. Patients were excluded if (1) they had a contraindication to nifedipine GITS according to the approved prescribing information; and (2) they were participating in an investigational program with interventions outside of routine clinical practice at the same time.

Procedures and Data Collection

Eligible patients received an oral dose of nifedipine GITS 60 mg and attended up to three clinic visits over a 12-week period. The timing of follow-up visits was not pre-specified and was according to the treating physician’s normal practice. Demographic and clinical data were collected from medical records, if available, or by interviewing the patient. Treatment-related data were collected during the initial visit and follow-up visits. Baseline and follow-up visit data for each patient were collected in the electronic case report form.

Study Outcomes and Endpoints

Effectiveness and safety were the outcomes assessed in this study. Effectiveness of nifedipine GITS 60 mg was evaluated through change in office SBP from baseline to week 12 and was considered as the primary endpoint of the study. The secondary endpoints for the study were change in DBP from baseline to week 12, changes in office SBP and DBP in different SBP subgroups (SBP 140–160 mmHg, ≥ 160 mmHg), changes in office SBP and DBP in subgroups of CKD stages (stage 1 [eGFR ≥ 90 mL/min/1.73 m2], stage 2 [eGFR 60–89 ml/min/1.73 m2], stage 3 [eGFR 30–59 mL/min/1.73 m2], stage 4 [eGFR 15–29 mL/min/1.73 m2), and stage 5 [eGFR < 15 mL/min/1.73 m2]), and the SBP and DBP control rate at week 12 (BP control goal was to maintain an average office SBP < 140 mmHg and DBP < 90 mmHg; maintain an average office SBP < 130 mmHg and DBP < 80 mmHg when urine albumin excretion rate was ≥ 30 mg/24 h). Safety outcomes were assessed by the incidence of adverse events (AEs) that were coded by the Medical Dictionary for Regulatory Activities (MedDRA) version 23.0. Information on AEs including treatment-emergent AEs (TEAEs), adverse drug reactions (ADRs), TEAEs leading to dosage adjustment, TEAEs leading to drug interruption, TEAEs leading to drug withdrawal, severe AEs (SAEs), drug-related SAEs, and TEAEs leading to death was summarized by the number of events, the number of subjects, and incidence rate.

Statistical Analysis

The statistical analysis of this study is of a descriptive nature. A total of 622 patients were included in the efficacy analysis set (EFF) that will provide the two-sided 95% confidence interval (CI) at ± 1.3 mmHg, which is considered to provide enough precision on the estimations of primary endpoint office SBP. Continuous data were described by the number of non-missing patients, number of missing patients (N miss), and mean. Continuous variables were described by the absolute value and change from baseline per analysis time point, if applicable. For categorical variables, the number and proportion of patients were calculated. The proportions were calculated on the basis of non-missing data. Sensitivity analysis was performed for the missing primary and secondary variables at week 12 by replacing them with the mean of the non-missing values at week 12. All statistical analyses were performed using SAS® version 9.4 (SAS Institute Inc., Cary, NC, USA).

Results

Patient Demographics

A total of 902 patients were screened, and 871 patients were enrolled on the basis of the inclusion and exclusion criteria. Out of the enrolled patients, 654 (72.5%) completed the study and 248 (27.5%) patients prematurely discontinued from the study (Fig. 1). All the enrolled 871 patients were included in the safety analysis set (SAF) and 622 (69.0%) were included in the EFF. The mean age of the study population was 50.9 years (range 19–73 years) and majority of them were male (66.8%). The baseline characteristics of the study population are provided in Table 1. The mean office SBP and DBP at baseline were 162.9 mmHg and 97.3 mmHg, respectively. The mean duration of hypertension (N = 537, N miss = 334) was 78.2 months. Out of 871 patients, 754 patients had CKD for 23.1 months on average and information for 117 patients were missing. Furthermore, 208 (24.4%), 194 (22.8%), 255 (29.9%), 185 (21.7%), and 10 (1.2%) patients had stage 1 (eGFR ≥ 90 mL/min/1.73 m2), stage 2 (eGFR 60–89 mL/min/1.73 m2), stage 3 (eGFR 30–59 mL/min/1.73 m2), stage 4 (eGFR 15–29 mL/min/1.73 m2), and stage 5 (eGFR < 15 mL/min/1.73 m2) CKD, respectively, with data missing for 19 patients. The underlying causes of CKD are presented in Table S2 in the supplementary material. The common comorbidities observed in patients were hyperlipidemia (44.8%), diabetes mellitus (32.1%), cardiovascular disease (12.5%), stroke (10.0%), and hyperuricemia (41.0%).

Fig. 1
figure 1

Flowchart representing patient disposition

Table 1 Baseline characteristics

Change in Office SBP from Baseline to Week 12

After 12 weeks of nifedipine GITS 60 mg treatment, patients showed a marked decrease in office SBP from baseline (Fig. 2). The mean SBP decreased from 162.9 mmHg at baseline to 138.9 mmHg at week 12. The mean change in SBP was − 24.0 mmHg (95% CI − 25.32, − 22.65 mmHg). After missing data were accounting for in sensitivity analysis, the mean change in SBP was − 23.9 mmHg (95% CI − 25.25, − 22.60 mmHg) at week 12 (Table 2).

Fig. 2
figure 2

Change in office SBP and DBP from baseline to week 12. BP blood pressure, DBP diastolic BP, SBP systolic BP

Table 2 Change in office SBP and DBP from baseline to week 12 (EFF)

Change in DBP from Baseline to Week 12

After 12 weeks of nifedipine GITS 60 mg treatment, there was a marked decrease in DBP from baseline (Fig. 2). The mean DBP decreased from 97.3 mmHg at baseline (N = 619) to 82.9 mmHg after 12 weeks (mean change − 14.3 mmHg, 95% CI − 15.27, − 13.37 mmHg). After missing values were accounted for by sensitivity analysis (N = 618), the mean change in DBP was − 14.3 mmHg (95% CI − 15.28, − 13.39 mmHg), suggesting that the analysis was robust (Table 2).

Change in SBP and DBP from Baseline to Week 12 in Different Patient Subgroups

Baseline SBP Subgroups

At week 12, the mean SBP decreased from 150.3 mmHg at baseline to 135.3 mmHg in the SBP 140–160 mmHg group, and decreased from 174.7 mmHg at baseline to 142.4 mmHg in the SBP ≥ 160 mmHg group; the mean changes were − 14.9 mmHg (95% CI − 16.20, − 13.68 mmHg) and − 32.3 mmHg (95% CI − 34.18, − 30.42 mmHg), respectively (Fig. 3). There was no change in mean change after accounting for missing data by sensitivity analyses. Furthermore, at week 12, the mean DBP decreased from 93.2 mmHg at baseline to 82.2 mmHg in the SBP 140–160 mmHg group and decreased from 101.1 mmHg at baseline to 83.7 mmHg in the ≥ 160 mmHg group; the mean changes were − 10.9 mmHg (95% CI − 12.08, − 9.73 mmHg) and − 17.4 mmHg (95% CI − 18.83, − 16.04 mmHg), respectively, and after missing data were accounted for, the mean changes were − 11.0 mmHg (95% CI − 12.15, − 9.82 mmHg) and − 17.4 mmHg (95% CI − 18.83, − 16.04 mmHg), confirming the robustness of the analysis (Table 3). The reduction of SBP and DBP was positively correlated with baseline BP.

Fig. 3
figure 3

Change in office SBP and DBP from baseline to week 12 in different patient subgroups. DBP diastolic blood pressure, SBP systolic blood pressure

Table 3 Change in SBP and DBP from baseline to week 12 in SBP subgroups (EFF)

Stage of CKD Subgroups

There was a marked decrease in SBP and DBP from baseline to week 12 irrespective of the CKD stage (Table S3 in the supplementary material). In patients with stage 1 CKD (N = 129), the mean SBP decreased from 160.4 mmHg at baseline to 136.9 mmHg at week 12 and the mean change in SBP was − 23.6 mmHg (95% CI − 26.28, − 20.91 mmHg). The mean DBP decreased from 96.6 mmHg at baseline to 82.9 mmHg at week 12 with a mean change of − 13.7 mmHg (95% CI − 15.48, − 11.92 mmHg). In patients with stage 2 CKD (N = 133), the mean SBP decreased from 163.3 mmHg at baseline to 137.2 mmHg at week 12 with a mean change of − 26.0 mmHg (95% CI − 29.23, − 22.85 mmHg). The mean DBP decreased from 97.3 mmHg at baseline to 83.1 mmHg at week 12 with a mean change of − 14.1 mmHg (95% CI − 16.30, − 11.91 mmHg). In patients with stage 3 CKD (N = 198), the mean SBP decreased from 163.1 mmHg at baseline to 139.0 mmHg at week with a mean change of − 24.2 mmHg (95% CI − 26.56, − 21.87 mmHg). Additionally, the mean DBP decreased from 97.7 mmHg at baseline to 82.6 mmHg at week 12 with a mean change of − 15.0 mmHg (95% CI − 16.9, − 13.12 mmHg). In patients with stage 4 CKD (N = 154), the mean SBP decreased from 164.3 mmHg at baseline to 142.1 mmHg at week 12 with a mean change of − 22.3 mmHg (95% CI − 25.00, − 19.51 mmHg) and the mean DBP decreased from 96.9 mmHg at baseline to 83.2 mmHg at week 12 accounting for a mean change of − 13.7 mmHg (95% CI − 15.43, − 12.00 mmHg) (Fig. 4). Sensitivity analysis revealed that the results of the subgroup analysis were robust irrespective of the missing data.

Fig. 4
figure 4

Change in office SBP from baseline to week 12 (EFF, stage of CKD subgroups). CKD chronic kidney disease, EFF efficacy analysis set, SBP systolic blood pressure

SBP and DBP Control Rate at Week 12

In the EFF, 49.7% and 50.3% of patients achieved SBP and DBP control goal, respectively, by analysis and sensitivity analyses, and the control rate (95% CI) was 50.0% (46.1%, 53.9%) by analysis and 50.3% (46.3%, 54.3%) by sensitivity analyses at week 12 (Table S4 in the supplementary material).

Adverse Events

All the enrolled patients (N = 871) were included in SAF, the mean (standard deviation) duration of exposure to the study drug was 89.2 (39.99) days. On the basis of SAF, 8.3% (72/871) of patients experienced 117 TEAEs, 2.6% (23/871) of patients experienced 23 ADRs, 2.2% (19/871) of patients experienced 20 TEAEs leading to drug interruption, and no TEAE leading to death was reported (Table S5 in the supplementary material). The observed ADRs were mainly dizziness (0.8%), generalized edema (0.6%), and headache (0.3%) (Table S6 in the supplementary material). We observed 16 SAEs in 1.7% (15/871) of patients (Table S7 in the supplementary material), and only 1 SAE (1/871, 1%, hyperkalemia) was considered as related to the study drug (no action was taken to the study drug and the event resolved on its own). No hypotension event was reported in any of the patients.

Discussion

CKD is a life-threatening disease that results in ESRD and consumes substantial health resources [20]. In China, the prevalence of CKD in the general population increases gradually with age [5]. Uncontrolled hypertension is a risk factor for developing CKD, and is the second leading cause of ESRD in the USA [21, 22]. Similarly, a decrease in eGFR leads to the exacerbation of uncontrolled hypertension due to volume expansion and increased systemic vascular resistance [4]. Multiple guidelines emphasize the importance of lowering BP to slow the progression of renal disease and reduce cardiovascular morbidity and mortality [23, 24]. Studies such as AASK and Rein-2 have confirmed the obvious benefits of stringent BP control in patients with CKD, which can reduce proteinuria, delay the decline of renal function, and improve the prognosis of patients with CKD [25, 26]. This emphasizes the need for effective antihypertensive treatment options to delay the progression of CKD. However, achieving BP control is a challenge in patients with CKD and uncontrolled hypertension [6, 7].

Studies have shown that with increase in dosage, the magnitude of lowering BP with CCB monotherapy also increases [18, 27]. Our study evaluated the effectiveness and tolerability of nifedipine GITS 60 mg in a relatively large cohort of Chinese patients with CKD and uncontrolled hypertension and filled the data gap for high-dose CCB treatment in Chinese patients with CKD. A multicenter study in China showed that only 33.1% and 14.1% patients with CKD and hypertension achieved the target BP < 140/90 and < 130/80 mmHg, respectively [6]. In our study, we enrolled patients with CKD and uncontrolled hypertension, and the BP control goal followed the guidelines’ recommendation: maintain an average office SBP/DBP < 140/90 mmHg, and maintain an average office SBP/DBP < 130/80 mmHg when urine albumin excretion was ≥ 30 mg/24 h. After 12 weeks of nifedipine GITS 60 mg treatment, we observed a remarkable reduction in office SBP and DBP with a control rate of 50.3%, which is much higher than the previous BP control rate in patients with CKD [6]. Achieving BP control is challenging in patients with CKD; the underlying cause for poor BP control might be due to physician’s unawareness of guidelines and therapeutic inertia together with poor medication adherence and lifestyle modifications by patients. In the future studies, healthcare providers and patient interventions need to be established to address these causes.

We also found some interesting outcomes in the subgroups analysis. In baseline SBP subgroups, we noted that mean change in SBP and DBP was related to the baseline SBP after 12 weeks nifedipine GITS 60 mg treatment; the higher the baseline SBP was, the greater the BP reduction. This finding is consistent with previous findings where patients with higher SBP at baseline and treated with nifedipine GITS reported greater reduction in BP than in patients with a lower baseline SBP [28, 29]. In CKD stage subgroups, we observed a marked decrease in office SBP and DBP with conspicuous mean changes at week 12 irrespective of the CKD stage, and the mean change in SBP/DBP of each stage was basically consistent with the overall results. This means that the antihypertensive effect of nifedipine 60 mg was not affected by CKD stage, which is a very important finding because we know that with successive CKD stages, the prevalence of hypertension in patients with non-dialysis CKD increases, but the control of hypertension decreases (P < 0.001) [6]. In a multicenter study conducted by Cai et al., elderly adults with CKD stage 4 (N = 408) and stage 5 (N = 748) had difficulty in achieving BP control compared with those with patients with CKD stage 1 (N = 342) (CKD stage 4, odds ratio [OR] = 0.5, P = 0.002; CKD stage 5, OR = 0.4; P < 0.001) [30]. Previously conducted studies have also demonstrated the effectiveness of nifedipine GITS 60 mg [19, 31, 32]. In the EXACT trail, a 20-week, post-marketing surveillance study of the effectiveness and patient tolerability of nifedipine GITS 30 or 60 mg, the final BP readings after 20 weeks of treatment in the 30-mg group (141.5 ± 0.4/84.8 ± 0.2 mmHg) and 60-mg group (146.6 ± 0.8/88.8 ± 0.4 mmHg) were significantly decreased from baseline. The study also indicated a better reduction in BP with nifedipine GITS 60 mg than GITS 30 mg [19]. The INSIGHT study showed the effectiveness of nifedipine GITS 30 mg in reducing BP from 173/99 mmHg to 138/82 mmHg [31]. Similarly, at week12, 30–60 mg/day nifedipine GITS significantly (P < 0.0001) reduced sitting SBP (17 ± 14 mmHg) and sitting DBP (14 ± 8 mmHg) in the MATH trial [32]. In another study, nifedipine GITS in doses of 30, 60, or 90 mg once daily was more effective than sustained-release propranolol in reducing standing (P < 0.005) and sitting SBP (P < 0.001) and DBP (P < 0.02) [33].

Nifedipine and other CCBs are associated with AEs such as edema, dizziness, and headache [34]. In previous studies, the rate of AE occurrence was low with nifedipine GITS 30 mg monotherapy. In the study by Ueng et al., 1.6% of the study population reported 286 AEs [34]. Nifedipine GITS 30 mg also reported a low rate of SAE occurrence. In our study, the patients treated with nifedipine GITS 60 mg showed great tolerability. ADRs were observed in 2.6% of patients and the most likely cause for these events may be associated with patients’ concurrent conditions/underlying diseases and concomitant medications being taken. No event of hypotension or death occurred during the study.

Overall, owing to the availability of a limited number of studies with nifedipine GITS 60 mg in the Chinese population, this evidence on nifedipine GITS 60 mg might play an essential role in improving the hypertension management practice in patients with CKD, and offer a new option. The main strength of the study is its prospective nature, which minimizes the recall bias. However, the study also has some limitations. This is a single-arm study without any comparator/control arm. Comparison with a lower dose group of nifedipine would have made a better evaluation of the efficacy and safety of nifedipine GITS 60 mg. Moreover, being an observational study, it might be subject to known and unknown confounders and biases.

Conclusion

The results of this prospective observational study showed that nifedipine GITS 60 mg is effective in reducing office SBP and DBP in Chinese patients with CKD and uncontrolled hypertension. The reduction of SBP/DBP was positively correlated with baseline BP and was not affected by different stages of CKD. In addition, analysis of the safety results revealed that nifedipine GITS 60 mg was safe and well tolerated in the studied population.