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AKAP12/Gravin is over-expressed in patients with ulcerative colitis

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Abstract

The gene of A-kinase anchor protein 12 (AKAP12) regulates cell cycle progression, cell motility, and morphology through its multiple scaffolding domains. However, the role of AKAP12 expression in ulcerative colitis (UC) patients has not been yet described. The aim of the study was to describe the gene and protein of AKAP12 expression in patients with UC and its association regarding the disease severity. We included a total of 40 patients with confirmed diagnosis of UC and 25 controls without endoscopic evidence of colitis or neoplasia. The relative quantification of the gene expression was performed by real-time PCR for AKAP12. Kruskal–Wallis was used to test differences among groups, and Spearman correlation to assess the relationship between AKAP12 gene and clinical outcomes. The extent of disease was evaluated using total colonoscopy, and biopsies were taken from rectum segments. The AKAP12 gene expression was increased in colonic mucosa from patients with active UC when compared with UC remission and control group. The overexpression of AKAP12 in patients with UC was associated with the presence of extensive colitis (p = 0.04, RM = 12, IC = 1.29–186.37). AKAP12/CD16 double positive cells were higher in submucosa (p = 0.04), muscular (p < 0.001), and cells from serosa (p < 0.001) in patients affected by UC in comparison to controls. The overexpression of AKAP12 was associated with the extent of disease. This is the first report about the role of AKAP12 in patients with UC suggesting that this gene and its protein could be involved in the modulation of the disease.

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Abbreviations

AKAP12:

A-kinase anchoring protein 12

aUC:

Active ulcerative colitis

cDNA:

Complementary DNA

DAB:

Diaminobenzidine

dNTPs:

Deoxyribonucleotide triphosphate

DSS:

Dextran sodium sulfate

EIMs:

Extra-intestinal manifestations

GADPH:

Glyceraldehyde-3-phosphate dehydrogenase

HRP:

Horseradish peroxidase

IBD:

Inflammatory bowel disease

MgCl2 :

Magnesium chloride

PCR:

Polymerase chain reaction

PKA:

Protein kinase A

RT:

Reverse transcription

RT-PCR:

Real-time polymerase chain reaction

rUC:

Remission ulcerative colitis

SD:

Standard deviation

SEM:

Standard error of the mean

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Funding

This work was supported by funds from the Inflammatory Bowel Disease Clinic at the National Institute of Medical Sciences and Nutrition.

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Authors and Affiliations

  1. Inflammatory Bowel Disease Clinic, Instituto Nacional de Ciencias Médicasy Nutrición Salvador Zubirán, Vasco de Quiroga #15, Col. Belisario Domínguez Sección XVI, CP, CDMX, 14080, Mexico City, Mexico

    Gabriela Fonseca-Camarillo & Jesús K. Yamamoto-Furusho

  2. Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, Mexico City, Mexico

    Janette Furuzawa-Carballeda

  3. Department of Internal Medicine, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, Mexico City, Mexico

    Ángel Alexis Priego-Ranero

  4. Department of Endoscopy, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, Mexico City, Mexico

    Rafael Barreto Zúñiga

  5. Department of Pathology, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, Mexico City, Mexico

    Braulio Martínez-Benítez

Authors
  1. Gabriela Fonseca-Camarillo
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  2. Janette Furuzawa-Carballeda
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  3. Ángel Alexis Priego-Ranero
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  4. Rafael Barreto Zúñiga
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  5. Braulio Martínez-Benítez
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  6. Jesús K. Yamamoto-Furusho
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Contributions

GFC participated during the sample processing, performed RT-qPCR analysis for AKAP12 transcript level quantification data analysis and prepared the manuscript, critical reviewing, and bibliographic analysis.

JFC participated during the sample processing, data analysis and prepared the manuscript, critical reviewing, and bibliographic analysis.

AAPR, RBZ, and BMB participated during the sample processing.

JKYF designed and provided the research idea, directed, reassessed both clinical and histological diagnostic, and coordinated the manuscript editing.

Corresponding author

Correspondence to Jesús K. Yamamoto-Furusho.

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The authors declare no competing interests.

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Fonseca-Camarillo, G., Furuzawa-Carballeda, J., Priego-Ranero, Á.A. et al. AKAP12/Gravin is over-expressed in patients with ulcerative colitis. Immunol Res 69, 429–435 (2021). https://doi.org/10.1007/s12026-021-09214-3

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  • DOI: https://doi.org/10.1007/s12026-021-09214-3

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