Abstract
Fungi have been reported as common inhabitants of the maritime waters in Antarctica by studies based on culture-dependent methods. More recently, results obtained using DNA sequencing technologies, revealed that fungal diversity worldwide has been underestimated by culture methods. The present study provides the first characterization of fungal communities in the coastal waters of King George Island (maritime Antarctica) using both culture-dependent and high-throughput sequencing (HTS) methods. HTS demostrated a higher level of fungal diversity than the obtained by culture methods. A high prevalence of basidiomycetous yeasts and ascomycetous filamentous fungi was confirmed by both methods, however, Chythriomycota, Rozellomycota, lichenized fungi and Malassezia spp. were detected only by HTS. Correspondingly, members of some genera, such as Metschnikowia, were only found by culture-dependent methods. Our results confirm that culturing and HTS, should be seen as complementary approaches that enable one to obtain a more comprehensive picture of the composition of microbial communities.
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We are grateful to the Comisión Sectorial de Investigación Científica (CSIC Uruguay); Instituto Antártico Uruguayo and Programa de Desarrollo de Ciencias Básicas (Pedeciba) who supported this work.
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GG, MW, SV conceived and designed the research. AA, AMS, RV conducted experiments. GG, MW, SV analyzed data and wrote the manuscript. All authors read and corrected the manuscript. All authors read and approved the final manuscript.
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Garmendia, G., Alvarez, A., Villarreal, R. et al. Fungal diversity in the coastal waters of King George Island (maritime Antarctica). World J Microbiol Biotechnol 37, 142 (2021). https://doi.org/10.1007/s11274-021-03112-4
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DOI: https://doi.org/10.1007/s11274-021-03112-4