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Plant growth promotion of micropropagated sugarcane seedlings var. Co 412 inoculated with endophytic diazotrophic bacteria and effects on the Ratoon Stunting Disease

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Abstract

The present study aimed to evaluate the influence of Gluconacetobacter diazotrophicus (PAL5), Herbaspirillum seropedicae (HRC54) and Herbaspirillum rubrisubalbicans (HCC103) on seedling vigor, plant-crop productivity and potential to control the sugarcane Ratoon Stunting Disease (RSD) caused by Leifsonia xyli subsp. xyli (Lxx). Micropropagated seedlings (variety Co 421) were inoculated with endophytic diazotrophic bacteria and transplanted to the nursery. Lxx was inoculated in seedlings 80 days before field planting (seven months) by pruning the basal leaves with scissors pre-immersed in contaminated sap. To estimate diazotrophic bacteria's population density, shoot and root samples of seedlings transplanted to the nursery were collected at 42 and 86 days after inoculation. Agronomic characteristics of sugarcane plants were evaluated at harvest (16 months). In general, there was a population reduction of the bacterial endophytes after 86 days, compared to 42 days after inoculation. G. diazotrophicus provided greater gains in tons of cane per hectare (TCH) (68.6%) and tons of Brix per hectare (59.1%) than the other treatments. Plants inoculated with diazotrophic bacteria and challenged with the pathogen (Lxx) showed a high incidence of Lxx seropositive in the stalks. However, an increase in yield of 18.83 and 19.09 TCH was noticed, respectively, related to control and Lxx inoculated treatment in the first harvest (or growing season). The treatment with H. rubrisubalbicans alone showed a low incidence of Lxx and positive agronomic yield performance, suggesting some effect on the xylem pathogen colonization for the variety Co 421.

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Acknowledgements

The authors would like to thank CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAPERJ (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro), UENF (Universidade Estadual do Norte Fluminense Darcy Ribeiro) and FENORTE (Fundação Estadual do Norte Fluminense) for the post-graduate scholarships granted and for logistical, human and material support in the development of the research project and, UFRRJ (Universidade Federal Rural do Rio de Janeiro) for the release of the first author to make his doctoral studies at UENF.

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  1. Laboratório de Entomologia E Fitopatologia, Centro de Ciências E Tecnologias Agropecuárias, Universidade Estadual Do Norte Fluminense Darcy Ribeiro, Campos Do Goytacazes/RJ, 28013-602, Brazil

    J. B. Carneiro Jr., L. M. Barroso, E. C. Ponte & S. F. Silveira

  2. Laboratório de Biologia Celular E Tecidual, Centro de Biociências E Biotecnologia, Universidade Estadual Do Norte Fluminense Darcy , Campos Do Goytacazes/RJ, 28013-602, Brazil

    F. L. Olivares

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  1. J. B. Carneiro Jr.
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  2. L. M. Barroso
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  3. F. L. Olivares
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  4. E. C. Ponte
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  5. S. F. Silveira
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This manuscript is original and has not been submitted for publication in another journal. This work is part of the doctoral thesis of Doctor Josil de Barros Carneiro Junior, under the guidance of Professor Silvaldo Felipe da Silveira, as required by the Graduate Program in Plant Production at UENF, in Campos dos Goytacazes, RJ, Brazil. The first author performed most of the experimental conduct, data collection and laboratory analysis. All authors contributed to the conception and design of this research and commented on the previous versions of the manuscript and approved the final format of the manuscript for this submission to the APP.

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Correspondence to S. F. Silveira.

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Carneiro, J.B., Barroso, L.M., Olivares, F.L. et al. Plant growth promotion of micropropagated sugarcane seedlings var. Co 412 inoculated with endophytic diazotrophic bacteria and effects on the Ratoon Stunting Disease. Australasian Plant Pathol. 50, 513–522 (2021). https://doi.org/10.1007/s13313-021-00813-0

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